Structural Characterization of Monosialo-, Disialo- and Trisialo-gangliosides by Negative Ion AP-MALDI-QIT-TOF Mass Spectrometry with MSn Switching
Structural Characterization of Monosialo-, Disialo- and Trisialo-gangliosides by Negative Ion AP-MALDI-QIT-TOF Mass Spectrometry with MSn Switching
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DOI：
10.1007/s11064-012-0735-z
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发表时间：
2012-06-01
影响因子：
4.4
通讯作者：
Suzuki, Akemi
中科院分区：
文献类型：
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作者：
Ito, Emi;Tominaga, Akio;Suzuki, Akemi
常压基质辅助激光解吸/电离（AP-MALDI）是一种非常方便的生物分子软电离技术，可使分析物在常压条件下而不是在高真空条件下进行。在这项研究中，AP-MALDI离子源与四极离子阱飞行时间（QIT-TOF）质谱仪耦合，能够进行MSn分析。我们将该系统应用于单胞脂苷GM1 （NeuAc）和GM2 (NeuAc)，双胞脂苷GD2 (NeuAc, NeuAc), GD1a （NeuAc, NeuAc）和GD1b （NeuAc, NeuAc）和三胞脂苷GT1a （NeuAc, NeuAc, NeuAc）的结构表征。在该系统中，MS、MS2和MS3的负离子质谱可以在2 s的周期内测量。结果表明，负离子模式MS、MS2和MS3谱为测定分子量、低聚糖序列和神经酰胺结构提供了充分的信息，并表明在MSn切换下保持分析物大气条件的AP-MALDI-QIT-TOF质谱法对分析各类含唾液酸的GSLs、神经节苷类化合物的结构是非常有用和方便的。
The atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) is a quite convenient soft ionization for biomolecules, keeping analytes atmospheric conditions instead of high vacuum conditions. In this study, an AP-MALDI ion source has been coupled to a quadrupole ion trap time-of-flight (QIT-TOF) mass spectrometer, which is able to perform MSn analysis. We applied this system to the structural characterization of monosialogangliosides, GM1 (NeuAc) and GM2 (NeuAc), disialogangliosides, GD2 (NeuAc, NeuAc), GD1a (NeuAc, NeuAc) and GD1b (NeuAc, NeuAc) and trisialoganglioside GT1a (NeuAc, NeuAc, NeuAc). In this system, the negative ion mass spectra of MS, MS2 and MS3, a set of three mass spectra, were able to measure within 2 s per cycle. Thus, obtained results demonstrate that the negative ion mode MS, MS2 and MS3 spectra provided sufficient information for the determination of molecular weights, oligosaccharide sequences and ceramide structures, and indicate that the AP-MALDI-QIT-TOF mass spectrometry keeping analytes atmospheric conditions with MSn switching is quite useful and convenient for structural analyses of various types of sialic acid-containing GSLs, gangliosides.