INTERACTION OF PYROPHOSPHATE MOIETIES WITH ALPHA-HELIXES IN DINUCLEOTIDE BINDING-PROTEINS
INTERACTION OF PYROPHOSPHATE MOIETIES WITH ALPHA-HELIXES IN DINUCLEOTIDE BINDING-PROTEINS
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DOI：
10.1021/bi00327a012
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
HOL, WGJ
中科院分区：
文献类型：
--
作者：
WIERENGA, RK;DEMAEYER, MCH;HOL, WGJ
研究了二核苷酸焦磷酸部分与那些螺旋在结合中起重要作用的蛋白质的结合。与 3 种不同二核苷酸相互作用的 6 种蛋白质的 3 维结构和氨基酸序列是可用的。由于谷胱甘肽还原酶结合 2 个二核苷酸，因此研究了 7 种酶-二核苷酸复合物。在所有这些复合物中，焦磷酸部分位于至少1个α-螺旋（二核苷酸结合螺旋）的N-末端附近。在二氢叶酸还原酶中，2 螺旋与 NADP 的焦磷酸相互作用。仅观察到所有复合物的2个共同特征：在螺旋的N末端出现甘氨酸以及α-螺旋偶极子与带负电的焦磷酸部分的有利相互作用。实际上在所有其他方面，二氢叶酸还原酶的二核苷酸结合都不同于其他 5 种蛋白质的结合模式。这 5 种蛋白质的螺旋被分组为 I 类二核苷酸结合螺旋。 6 个 I 类螺旋形成紧凑的 .beta..alpha..beta 的一部分。结构高度相似但序列非常不同的单元。可以推导出该单元的序列的特征指纹。对于这些紧凑的β..α..β，仅二核苷酸的ADP部分占据非常相似的位置。单位。这些单位的适当名称是 ADP 结合 .beta..alpha..beta。褶皱。
The binding of dinucleotide pyrophosphate moieties to those proteins where helixes play an important role in the binding was studied. The 3-dimensional structures and amino acid sequences of 6 proteins interacting with 3 different dinucleotides were available. As glutathione reductase binds 2 dinucleotides, 7 enzyme-dinucleotide complxes were studied. In all these complexes the pyrophosphate moiety is located near the N-terminus of at least 1 .alpha.-helix, the dinucleotide binding helix. In dihydrofolate reductase 2 helixes interact with the pyrophosphate of NADP. Only 2 common characteristics of all complxes are observed: the occurrence of a glycine at the N-terminus of the helix and the favorable interaction of the .alpha.-helix dipole with the negatively charged pyrophosphate moiety. In virtually every other respect, the dinucleotide binding by dihydrofolate reductase differs from the mode of binding by the 5 other proteins. The helixes of these 5 proteins were grouped together as category I dinucleotide binding helixes. The 6 category I helixes form part of a compact .beta..alpha..beta. unit of highly similar structure and very dissimilar sequences. A characteristic fingerprint for the sequence of this unit can be deduced. Only the ADP moieties of the dinucleotides occupy very similar positions with respect to these compact .beta..alpha..beta. units. An appropriate name for these units would be ADP binding .beta..alpha..beta. folds.