Detection of H-2 antigens on 8-cell mouse embryos.
Detection of H-2 antigens on 8-cell mouse embryos.
复制标题
8 细胞小鼠胚胎上 H-2 抗原的检测。
DOI：
10.1002/jez.1402210212
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Warner,CM
中科院分区：
文献类型：
--
作者：
Cozad,KM;Warner,CM
H - 2抗原在b、k和d单倍型的8细胞小鼠胚胎中检测到，但在a单倍型的8细胞胚胎中未检测到。用于检测胚胎上H - 2抗原的技术是一种高度敏感、自动化、补体依赖性的细胞毒性试验，其原理是活胚胎将3h -胸苷结合到DNA中，而用抗血清和补体杀死的胚胎则不会。8细胞胚胎中H - 2的含量明显低于囊胚。提出了H‐2抗原在发育中的可能功能。
H‐2 antigens were detected on 8‐cell mouse embryos of the b, k, and d haplotypes, but not on 8‐cell embryos of the a haplotype. the technique used for detection of H‐2 antigens on the embryos was a highly sensitive, automated, complement‐dependent cytotoxicity assay, based on the principle that live embryos will incorporate3H‐thymidine into DNA, but embryos killed with antiserum and complement will not. The amount of H‐2 present on 8‐cell embryos is apparently less than that present on blastocysts. A possible function of H‐2 antigens in development is proposed.