INTESTINAL RESISTANCE IN EXPERIMENTAL ENTERIC INFECTION OF MICE WITH A MOUSE ADENOVIRUS .1. GROWTH OF VIRUS AND APPEARANCE OF A NEUTRALIZING SUBSTANCE IN INTESTINAL TRACT
INTESTINAL RESISTANCE IN EXPERIMENTAL ENTERIC INFECTION OF MICE WITH A MOUSE ADENOVIRUS .1. GROWTH OF VIRUS AND APPEARANCE OF A NEUTRALIZING SUBSTANCE IN INTESTINAL TRACT
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DOI：
10.1111/j.1348-0421.1970.tb00538.x
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发表时间：
1970-01-01
期刊：
影响因子：
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通讯作者：
SASAKI, S
中科院分区：
文献类型：
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作者：
HASHIMTO, K;SUGIYAMA, T;SASAKI, S
本文研究了小鼠腺病毒K87株对近交系DK 1小鼠肠道的感染过程及感染后获得的肠道抵抗力。用荧光抗体技术计数感染肠道大部分含病毒抗原的细胞，并在小鼠肾组织培养中检测各部分病毒的感染性滴度。经口攻毒后3 ~ 14天（有时3 ~ 21天）病毒开始生长，感染性滴度在7 ~ 14天后达到高峰，此时检测到大量含病毒抗原的细胞和具有核包涵体的细胞。在初次攻击后28天再次攻击的小鼠中，病毒没有生长，也没有发现含有病毒抗原的细胞，由此可以得出结论，病毒在小鼠中生长的主要部位是分散在小肠粘膜上皮层中的细胞，这些细胞似乎是通常的上皮细胞，而不是潘氏细胞或杯状细胞。关于肠道抗性，进行了灭活疫苗和血清抗体被动转移实验，以确定血清中的中和抗体是否对肠壁中病毒的生长有任何影响，结果表明没有影响。攻毒后18天或更长时间，在感染小鼠的肠壁和肠内容物中检测到K87病毒中和物质，但在血清转移小鼠中未检测到，尽管两组小鼠的血清抗体水平相同。该物质持续存在于肠内容物中，直至攻毒后15周，并在肠壁中持续存在，直至晚于34周。讨论了该物质的性质和可能的作用，但实际数据将在随后的文件中报告。
Investigation was made on the process of enteric infection with mouse adenovirus strain K87 in inbred DK1 mice and the intestinal resistance acquired through infection. The cells containing viral antigens were enumerated in most parts of the infected intestinal tract by a fluorescent antibody technique, and the infectivity titer of the virus in each part was examined in mouse kidney tissue culture. The virus was observed to grow in 3∼ 14 days (sometimes 3∼ 21 days) after oral challenge, and infectivity titers reached their peak after 7∼ 14 days, when a number of viral antigen-containing cells and cells with nuclear inclusions were detected. In the mice rechallenged 28 days after the initial challenge, the virus did not grow, and no viral antigen-containing cells were found. From these results it was concluded that the main sites where the virus grows in mice are the cells which are scattered in the epithelial layer of the mucous membrane of the small intestine, and which seem to be the usual epithelial cells and not Paneth's or goblet cells. As for intestinal resistance, experiments with inactivated vaccine and with passive transfer of serum-antibodies were performed in order to find out whether neutralizing antibodies in the serum had any influence on the growth of virus in the intestinal wall, and no influences were indicated. Eighteen days or more after challenge, K87 virus-neutralizing substances were detected in the intestinal wall and in the intestinal contents of the infected mice, but not in the serum-transferred mice, though both groups of mice had equal levels of serum antibodies. The substance continued to be found until 15 weeks after challenge in the intestinal contents, and until later than 34 weeks in the intestinal walls. The nature and the possible role of the substance is discussed, but actual data will be reported in subsequent papers.