Postoperative pain and analgesic responses are similar in male and female Sprague-Dawley rats
Postoperative pain and analgesic responses are similar in male and female Sprague-Dawley rats
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DOI：
10.1007/bf03018737
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发表时间：
2003-11-01
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影响因子：
--
通讯作者：
Tuman, KJ
中科院分区：
文献类型：
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作者：
Kroin, JS;Buvanendran, A;Tuman, KJ
目的：在动物和人类研究中，关于性别对疼痛敏感性和止痛药反应的影响存在争议。本研究比较了雄性和雌性大鼠的术后疼痛评分，以及它们对止痛干预的反应。方法：在建立的术后疼痛模型上，在Sprague Dawley大鼠一侧足底切开，造成机械性超敏。术后4天监测疼痛评分，以确定两组之间的内在差异。实验动物于术后第2天全身及鞘内注射阿片类药物、α(2)-肾上腺素能激动剂、乙酰胆碱酯酶抑制剂、加巴喷丁，以评价这些药物抗痛觉过敏作用的性别差异。结果：在足底切开术后疼痛模型中，术后4d的机械超敏反应(von Frey细丝)无性别差异。腹腔注射吗啡(3 mg·kg~(-1))和加巴喷丁(25 mg·kg~(-1))可降低术后机械超敏反应，但无性别差异。鞘内注射吗啡(1-2nmol)、加巴喷丁(60-120nmol)、可乐定(45nmol)和新斯的明(6.6nmol)在镇痛效果上也没有性别差异。结论：本研究结果表明，SpragueDawley大鼠在术后疼痛感知和对止痛剂的反应方面没有性别差异，表明该品系的大鼠可以在术后疼痛研究中使用，而不会引入性别偏见。
Purpose: Controversy exists concerning the influence of gender on pain sensitivity and response to analgesics both in animal and human studies. The present study compares postoperative pain scores in male and female rats and how they respond to analgesic interventions.Methods: Unilateral plantar foot incisions were made in SpragueDawley rats of both genders, producing mechanical allodynia in an established model of postoperative pain. Postoperative pain scores were monitored for four days following incision to identify intrinsic differences between the two groups. Animals were tested with analgesics (opioids, alpha(2)-adrenergic agonists, acetylcholine esterase inhibitors, gabapentin) both systemically and intrathecally on the day after incision to assess gender differences in the anti-allodynic effect of these drugs.Results: In the plantar foot incision model of postoperative pain there was no gender difference in postoperative mechanical hypersensitivity (von Frey filaments) over four days. Morphine (3 mg(.)kg(-1)) and galbapentin (25 mg(.)kg(-1)) administered intraperitoneally decreased postoperative mechanical hypersensitivity, but with no gender difference. Intrathecal morphine (1-2 nmol), galbapentin (60-120 nmol), clonidine (45 nmol), and neostigmine (6.6 nmol) also showed no gender difference in analgesic effect.Conclusion: The results of this study demonstrate that in SpragueDawley rats there are no gender differences in postoperative pain perception or the response to analgesics, indicating that this strain of rats can be used without introducing gender bias in studies of postoperative pain.