CLASS-I HLA MOLECULES ON HUMAN-ERYTHROCYTES - QUANTITATION AND TRANSFUSION EFFECTS
CLASS-I HLA MOLECULES ON HUMAN-ERYTHROCYTES - QUANTITATION AND TRANSFUSION EFFECTS
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DOI：
10.1097/00007890-198707000-00025
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发表时间：
1987-07-01
期刊：
影响因子：
6.2
通讯作者：
SCORNIK, JC
中科院分区：
文献类型：
--
作者：
EVERETT, ET;KAO, KJ;SCORNIK, JC
HLA I类分子在表达高或低水平HLA I类抗原的个体的红细胞上进行定量分析。在竞争结合实验中，使用抗hla单克隆抗体W6/32的125i标记Fab片段完成定量测定。该测试系统的实验条件是利用流式细胞术检测发现表达高水平红细胞HLA的个体的红细胞建立的。竞争性结合实验满足配体特异性和特异性结合饱和度的要求。Scatchard分析显示有1684个+-。39（平均值+-）SD) HLA分子/红细胞。在另外两个供者中，流式细胞术检测不到红细胞HLA，明确显示125I-W6/32 Fab片段的特异性结合，表明HLA也存在于这些供者的红细胞上。据估计，这些人的HLA分子/红细胞数量在100到200之间。因此，在输血单位中，红细胞贡献的HLA分子数量与白细胞的数量相当。白细胞和血小板高度耗尽的血液以及从红细胞HLA含量低的供体中选择的血液是无益的（当输注给选定的患者时），因为它们的致敏作用与常规输血没有显着差异。这些结果表明，红细胞上HLA抗原的数量，虽然与其他细胞类型相比较低，但在输血的绝对抗原含量方面是显著的。他们还表明，输血含有HLA抗原的血液单位，其浓度与现有技术所能达到的一样低，对于预防高危患者的HLA致敏没有作用。
HLA class I molecules were quantitated on erythrocytes from individuals expressing either high or low levels of such antigens. Quantitative determinations were accomplished using 125I-labeled Fab fragments of the anti-HLA monoclonal antibody W6/32 in a competitive binding assay. The experimental conditions of the test system were established using red cells from an individual found to express high levels of red cell HLA when examined by flow cytometry. The competitive binding assay met the requirements of ligand specificity and specific binding saturability. Scatchard analysis revealed that there were 1684 .+-. 39 (mean .+-. SD) HLA molecules/red cell. In two other donors in whom erythrocyte HLA was undetectable by flow cytometry specific binding of the 125I-W6/32 Fab fragments was clearly demonstrated, indicating the presence of HLA on red cells of these donors as well. The number of HLA molecules/red cell was estimated to be between 100 and 200 for these invididuals. Thus, in a blood transfusion unit, the number of HLA molecules contributed by the red cells is comparable to that of the leukocytes. Blood highly depleted of leukocytes and platelets and selected from donors with low amounts of red cell HLA was not beneficial (when transfused to selected patients) in that their sensitizing effects were not significantly different from regular blood transfusions. These results show that the amount of HLA antigens on red cells, while low if compared with other cell types, is significant in terms of the absolute antigenic content of blood transfusions. They also show that transfusion of blood units containing HLA antigens in concentrations as low as can be achieved with current technology were not useful in preventing HLA sensitization in patients at risk.