Airway Exposure to Modified Multi-walled Carbon Nanotubes Perturbs Cardiovascular Adenosinergic Signaling in Mice.
Airway Exposure to Modified Multi-walled Carbon Nanotubes Perturbs Cardiovascular Adenosinergic Signaling in Mice.
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气道暴露于改良的多壁碳纳米管中，小鼠心血管腺苷能信号传导。
DOI：
10.1007/s12012-018-9487-6
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发表时间：
2019-04
影响因子：
3.2
通讯作者：
Wingard CJ
中科院分区：
文献类型：
--
作者：
Thompson LC;Sheehan NL;Walters DM;Lust RM;Brown JM;Wingard CJ
多壁碳纳米管（MWCNT）的广泛商业应用可以通过添加各种表面化学改性来进一步扩展。例如，标准商业级MWCNT（C级）可以被羧化（COOH）或氮掺杂（N掺杂）以适合特定用途。我们先前报道了在小鼠体内注入C级、COOH或N掺杂的多壁碳纳米管后24小时，心脏缺血/再灌注（I/R）损伤的剂量依赖性扩大。在这里，我们已经测试的假设，气道暴露于多壁碳纳米管干扰心血管腺苷能信号，这可能会导致心脏I/R损伤的恶化。将100微升的媒介物或含有100 μg C级、COOH或N掺杂的MWCNT的相同悬浮液体积滴入CD-1 ICR小鼠的气管中。一天后，我们测量了心脏组织中的环磷酸腺苷（cAMP）浓度，并在离体主动脉组织中评价了与一氧化氮合酶（NOS）和环氧合酶（考克斯）相关的动脉腺苷能平滑肌信号传导机制。我们还验证了心脏I/R损伤扩大，并检查了肺组织学和支气管肺泡灌洗液细胞在多壁碳纳米管暴露的小鼠。与媒介物组相比，心肌cAMP浓度在C级组中降低17.4%（p < 0.05），在N掺杂组中降低13.7%（p < 0.05）。与媒介物组相比，MWCNT组中主动脉环2-Cl-腺苷浓度响应的曲线拟合显著更大。抑制NOS时主动脉收缩反应更明显，而抑制考克斯时主动脉收缩反应消失。这些研究结果表明，在多壁碳纳米管的表面上添加功能性化学部分可能会改变暴露的生物反应，通过影响心血管腺苷能信号传导和促进心脏损伤。
The broad list of commercial applications for multi-walled carbon nanotubes (MWCNT) can be further expanded with the addition of various surface chemistry modifications. For example, standard commercial grade MWCNT (C-grade) can be carboxylated (COOH) or nitrogen-doped (N-doped) to suite specific utilities. We previously reported dose-dependent expansions of cardiac ischemia/reperfusion (I/R) injury, 24 hours after intratracheal instillation of C-grade, COOH, or N-doped MWCNT in mice. Here we have tested the hypothesis that airway exposure to MWCNT perturbs cardiovascular adenosinergic signaling, which could contribute to exacerbation of cardiac I/R injury. One hundred microliters of Vehicle or identical suspension volumes containing 100 μg of C-grade, COOH, or N-doped MWCNT were instilled into the trachea of CD-1 ICR mice. One day later we measured cyclic adenosine monophosphate (cAMP) concentrations in cardiac tissue and evaluated arterial adenosinergic smooth muscle signaling mechanisms related to nitric oxide synthase (NOS) and cyclooxygenase (COX) in isolated aortic tissue. We also verified cardiac I/R injury expansion and examined both lung histology and bronchoalveolar lavage fluid cellularity in MWCNT exposed mice. Myocardial cAMP concentrations were reduced (p < 0.05) in the C-grade group by 17.4% and N-doped group by 13.7% compared to the Vehicle group. Curve fits to aortic ring 2-Cl-Adenosine concentration responses were significantly greater in the MWCNT groups vs. the Vehicle group. Aortic constrictor responses were more pronounced with NOS inhibition and were abolished with COX inhibition. These findings indicate that addition of functional chemical moieties on the surface of MWCNT may alter the biological responses to exposure by influencing cardiovascular adenosinergic signaling and promoting cardiac injury.
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由多壁碳纳米管暴露引起的MMP-9依赖性血清传播生物活性通过CD36清道夫受体诱导血管功能障碍。
DOI：
10.1093/toxsci/kfw015
发表时间：
2016-04
期刊：
Toxicological sciences : an official journal of the Society of Toxicology
影响因子：
--
作者：
Aragon M;Erdely A;Bishop L;Salmen R;Weaver J;Liu J;Hall P;Eye T;Kodali V;Zeidler-Erdely P;Stafflinger JE;Ottens AK;Campen MJ
通讯作者：
Campen MJ
DOI：
10.1152/ajpheart.00764.2007
发表时间：
2007-12-01
影响因子：
4.8
作者：
Ansari, Habib R.;Nadeem, Ahmed;Mustafa, S. Jamal
通讯作者：
Mustafa, S. Jamal
影响因子：
10.4
作者：
Bonner JC;Silva RM;Taylor AJ;Brown JM;Hilderbrand SC;Castranova V;Porter D;Elder A;Oberdörster G;Harkema JR;Bramble LA;Kavanagh TJ;Botta D;Nel A;Pinkerton KE
通讯作者：
Pinkerton KE
影响因子：
10.8
作者：
ACHTERBERG, PW;HARMSEN, E;DEJONG, JW
通讯作者：
DEJONG, JW
影响因子：
15.9
作者：
FOX, AC;REED, GE;SILK, BB
通讯作者：
SILK, BB