Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus
Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus
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DOI：
10.1186/1471-2164-8-462
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发表时间：
2007-12-18
期刊：
影响因子：
4.4
通讯作者：
Chen, Cheng-Chen
中科院分区：
文献类型：
--
作者：
Mayhew, George F.;Bartholomay, Lyric C.;Chen, Cheng-Chen
背景资料：当感染了马来丝虫（一种淋巴丝虫病的病原体）时，蚊子，即白纹阿蚊，会产生一种非常强大的先天免疫反应。为了挖掘转录组以获得对这种蚊子中响应感染而发生的级联事件的新见解，从经历免疫应答激活处理的成年雌性蚊子的组织和从老化的幼稚蚊子产生6个cDNA文库，所述免疫应答激活处理导致充分表征的应答。结果：构建了6个文库，共获得44，940个表达序列标签，其中38，079个通过了质量筛选，被纳入注释项目和后续分析。所有这些序列被折叠成簇，产生8，020个独特序列簇或单例。EST聚类在ASAP中进行注释和人工管理（A Systematic Annotation Package for Community Analysis of Genomes）门户网站根据BLAST结果与Genbank、冈比亚按蚊和黑腹果蝇基因组计划进行比较。由此产生的数据集是该蚊子媒介的第一个此类数据集，并为未来研究蚊子媒介中发生的级联事件提供了基础。感染的反应，从而提供深入了解载体的能力和先天免疫。
Background: The mosquito, Armigeres subalbatus, mounts a distinctively robust innate immune response when infected with the nematode Brugia malayi, a causative agent of lymphatic filariasis. In order to mine the transcriptome for new insight into the cascade of events that takes place in response to infection in this mosquito, 6 cDNA libraries were generated from tissues of adult female mosquitoes subjected to immune-response activation treatments that lead to well-characterized responses, and from aging, naive mosquitoes. Expressed sequence tags (ESTs) from each library were produced, annotated, and subjected to comparative analyses.Results: Six libraries were constructed and used to generate 44,940 expressed sequence tags, of which 38,079 passed quality filters to be included in the annotation project and subsequent analyses. All of these sequences were collapsed into clusters resulting in 8,020 unique sequence clusters or singletons. EST clusters were annotated and curated manually within ASAP (A Systematic Annotation Package for Community Analysis of Genomes) web portal according to BLAST results from comparisons to Genbank, and the Anopheles gambiae and Drosophila melanogaster genome projects.Conclusion: The resulting dataset is the first of its kind for this mosquito vector and provides a basis for future studies of mosquito vectors regarding the cascade of events that occurs in response to infection, and thereby providing insight into vector competence and innate immunity.