FREE AMINO-ACIDS IN BIOPSY SPECIMENS FROM THE HUMAN COLONIC MUCOSA
FREE AMINO-ACIDS IN BIOPSY SPECIMENS FROM THE HUMAN COLONIC MUCOSA
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DOI：
10.1006/jsre.1993.1198
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发表时间：
1993-12-01
影响因子：
2.2
通讯作者：
WERNERMAN, J
中科院分区：
文献类型：
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作者：
AHLMAN, B;LEIJONMARCK, CE;WERNERMAN, J
对10例患者的直肠、降结肠、横结肠和升结肠的内镜活检标本进行游离氨基酸和总蛋白含量的测定。用离子交换色谱法测定氨基酸含量，用荧光法测定氨基酸含量。与血浆相比，结肠中的氨基酸模式和单个氨基酸的等级顺序有所不同。谷氨酸、牛磺酸、天冬氨酸、谷氨酰胺、甘氨酸、丙氨酸、丝氨酸、赖氨酸、缬氨酸和鸟氨酸是结肠中含量最多的10种氨基酸。在冒号的所有不同部分中，排列顺序是相同的。活组织检查越迟，氨基酸的浓度越低。直肠蛋白质含量显著低于横结肠，其余各节段之间无显著差异。该研究证明了从人类结肠粘膜的内镜活检中确定游离氨基酸的可能性，从降结肠和/或直肠/乙状结肠的活检标本可以被认为是整个大肠的代表。该技术可用于肠黏膜氨基酸代谢的重复采样研究。
The content of free amino acids and total protein was determined in endoscopic biopsy specimens from the rectum, descending colon, transverse colon, and ascending colon in 10 patients. The amino acids were quantified by ion-exchange chromatography and were detected by fluorescence. The amino acid pattern and the rank order of the individual amino acids in the colon were different compared to those in plasma. Glutamate, taurine, aspartate, glutamine, glycine, alanine, serine, lysine, valine, and ornithine were the 10 most abundant amino acids in the colon. The rank order was the same in all of the different segments of the colon. The concentrations of the amino acids decreased the more aborally the biopsies were taken. The protein content in the rectum was significantly lower than that in the transverse colon, but there were no difference between the different segments otherwise. The study demonstrated the possibility of determining free amino acids from endoscopic biopsies of human colonic mucosa, Biopsy specimens from the descending colon and/or rectum/sigmoid colon may be considered representative of the entire large intestine. The technique may be used for repeated sampling in studies of the amino acid metabolism of the intestinal mucosa.