Purification, crystallization and initial X-ray diffraction study of human REV7 in complex with a REV3 fragment
Purification, crystallization and initial X-ray diffraction study of human REV7 in complex with a REV3 fragment
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DOI：
10.1107/s1744309109046181
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发表时间：
2009-12-01
影响因子：
0.9
通讯作者：
Hashimoto, Hiroshi
中科院分区：
文献类型：
--
作者：
Hara, Kodai;Shimizu, Toshiyuki;Hashimoto, Hiroshi
REV7 参与多种细胞功能，包括 DNA 复制、信号转导和细胞周期调节。在 DNA 复制中，REV7 与 REV3 相互作用并形成 DNA 聚合酶 zeta，它在容易出错的 DNA 合成中发挥着核心作用。 REV3 是一个催化亚基，其活性受 REV7 刺激。为了阐明 REV7 和 REV3 之间相互作用的结构基础，将人 REV7 与 REV3 片段形成复合物结晶。获得两种晶型。晶型I和II属于空间群P2(1)，晶胞参数a = 43.8，b = 50.0，c = 107.3埃，β = 96.9度，以及P4(1)2(1)2或P4(3)2(1)2，晶胞参数a = b = 76.6，c = 118.4埃。
REV7 is involved in various cellular functions including DNA replication, signal transduction and cell-cycle regulation. In DNA replication, REV7 interacts with REV3 and forms DNA polymerase zeta, which plays a central role in error-prone DNA synthesis. REV3 is a catalytic subunit and its activity is stimulated by REV7. To clarify the structural basis of the interaction between REV7 and REV3, human REV7 was crystallized in complex with a REV3 fragment. Two crystal forms were obtained. Crystal forms I and II belonged to space groups P2(1), with unit-cell parameters a = 43.8, b = 50.0, c = 107.3 angstrom, beta = 96.9 degrees, and P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 76.6, c = 118.4 angstrom, respectively.