Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain.
Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain.
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DOI：
10.1186/1754-6834-7-44
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发表时间：
2014-03-26
影响因子：
6.3
通讯作者：
Xiao D
中科院分区：
文献类型：
--
作者：
Guo X;Cao C;Wang Y;Li C;Wu M;Chen Y;Zhang C;Pei H;Xiao D
2，3-丁二醇（2，3-BD）是一种高价值的化学品，通常由石油化工生产，但也可以由一些细菌合成。到目前为止，肺炎克雷伯氏菌是最强大的2，3-BD生产商，可以利用广泛的底物。然而，许多副产物也由K产生。肺炎杆菌的代谢产物，如乙醇、乳酸盐和乙酸盐，其负调节2，3-BD产率并增加下游分离和纯化的成本。在这项研究中，我们构建了K。通过自杀载体偶联，将具有乳酸脱氢酶（LDH）、乙醛脱氢酶（ADH）和磷酸转乙酰酶（PTA）缺失的肺炎链球菌突变体分别与细胞共培养。这些突变体表现出不同的生产形成行为。敲除ldhA对2，3-BD的产量影响不大，而敲除adhE或pta则显著提高了2，3-BD的产量。所有突变体中2，3-BD生物合成中间产物乙偶姻的积累均减少。然后在五种不同的碳源中测试突变体，并观察到增加的2，3-BD。同时构建了一株adhE和ldhA缺失的双突变菌株，该菌株的发酵速度加快，2，3-BD产量提高。在流加培养中，该菌株的2，3-BD产率达到0.49g/g，葡萄糖转化率超过100 g/L。随着adhE和pta的失活，2，3-BD的产量显著提高。ldhA的失活可促进细胞生长，缩短发酵时间。adhE和ldhA缺失的双突变菌株导致加速发酵和更高的2，3-BD产量。这些结果为K.肺炎。
2,3-Butanediol (2,3-BD) is a high-value chemical usually produced petrochemically but which can also be synthesized by some bacteria. To date, Klebsiella pneumoniae is the most powerful 2,3-BD producer which can utilize a wide range of substrates. However, many by-products are also produced by K. pneumoniae, such as ethanol, lactate, and acetate, which negatively regulate the 2,3-BD yield and increase the costs of downstream separation and purification. In this study, we constructed K. pneumoniae mutants with lactate dehydrogenase (LDH), acetaldehyde dehydrogenase (ADH), and phosphotransacetylase (PTA) deletion individually by suicide vector conjugation. These mutants showed different behavior of production formation. Knock out of ldhA had little influence on the yield of 2,3-BD, whereas knock out of adhE or pta significantly improved the formation of 2,3-BD. The accumulation of the intermediate of 2,3-BD biosynthesis, acetoin, was decreased in all the mutants. The mutants were then tested in five different carbon sources and increased 2,3-BD was observed. Also a double mutant strain with deletion of adhE and ldhA was constructed which resulted in accelerated fermentation and higher 2,3-BD production. In fed-batch culture this strain achieved more than 100 g/L 2,3-BD from glucose with a relatively high yield of 0.49 g/g. 2,3-BD production was dramatically improved with the inactivation of adhE and pta. The inactivation of ldhA could advance faster cell growth and shorter fermentation time. The double mutant strain with deletion of adhE and ldhA resulted in accelerated fermentation and higher 2,3-BD production. These results provide new insights for industrial production of 2,3-BD by K. pneumoniae.
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影响因子：
5
作者：
Petrov, Kaloyan;Petrova, Penka
通讯作者：
Petrova, Penka
DOI：
10.1007/bf00338087
发表时间：
1986-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
CUNNINGHAM, PR;CLARK, DP
通讯作者：
CLARK, DP
通过 NADH 氧化酶异源表达进行辅因子工程及其对肺炎克雷伯菌代谢通量重新分布的影响
影响因子：
6.3
作者：
Ji XJ;Xia ZF;Fu NH;Nie ZK;Shen MQ;Tian QQ;Huang H
通讯作者：
Huang H
影响因子：
5
作者：
Seo, Mi-Young;Seo, Jeong-Woo;Kim, Chul Ho
通讯作者：
Kim, Chul Ho
通过乙醛脱氢酶基因的插入失活改造产酸克雷伯菌以高效生产 2, 3-丁二醇
影响因子：
5
作者：
Ji, Xiao-Jun;Huang, He;Li, Shuang
通讯作者：
Li, Shuang