A non-invasive stress assay based upon measurement of free cortisol released into the water by rainbow trout
A non-invasive stress assay based upon measurement of free cortisol released into the water by rainbow trout
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DOI：
10.1111/j.0022-1112.2004.00499.x
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发表时间：
2004-11-01
影响因子：
2
通讯作者：
Scott, AP
中科院分区：
文献类型：
--
作者：
Ellis, T;James, JD;Scott, AP
一种以前用于性激素的程序被改造成从彩虹鲑鱼的水样中提取游离皮质醇和皮质醇。两种皮质类固醇激素均可用放射免疫分析法(RIA)检测到，其中皮质醇占主导地位。采样、提取和RIA程序的所有阶段都对皮质醇进行了验证。一个间歇性的重复性差的问题被追溯到提取过程中使用乙醚，并通过使用乙酸乙酯克服了这一问题。还引入了其他修改，以加快程序。这两种皮质类固醇的释放浓度和时间进程被证明与鱼类受到的压力程度有关。已证实，水中的皮质醇浓度和估计的皮质醇释放率随着处理压力的增加而增加，并且两者都与血浆皮质醇浓度相关。讨论了使用水中皮质醇浓度和释放率来评估鱼的初级应激反应作为血液采样的非侵入性替代方法的可能性。(C)2004年官方版权。
A procedure previously used for sex steroids was adapted to extract free cortisol and cortisone from water samples taken from rainbow trout Oncorhynchus mykiss ranks. Both corticosteroids Could be readily detected by radioimmunoassay (RIA), with cortisol being predominant. All stages of the sampling, extraction and RIA procedure were validated for cortisol. An intermittent problem with poor replication was traced to the use of diethyl ether during the extraction procedure, and was overcome by the use of ethyl acetate. Other modifications were also introduced to speed up the procedure. The concentration and time course of release of both corticosteroids were shown to be related to the degree of stress that the fish had been Subjected to. It was confirmed that cortisol concentrations in water and estimated cortisol release rates increased in response to handling stress, and that both were correlated with plasma cortisol concentrations. The potential for using water cortisol concentration and release rates to assess the primary stress response of fishes as a non-invasive alternative to blood sampling is discussed. (C) 2004 Crown copyright.