Effects of corticotropin-releasing factor on plasticity of optically recorded neuronal activity in the substantia gelatinosa of rat spinal cord slices
Effects of corticotropin-releasing factor on plasticity of optically recorded neuronal activity in the substantia gelatinosa of rat spinal cord slices
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DOI：
10.1016/j.pain.2003.08.004
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发表时间：
2003-11-01
期刊：
影响因子：
7.4
通讯作者：
Murase, K
中科院分区：
文献类型：
--
作者：
Ikeda, H;Kusudo, K;Murase, K
我们研究了促肾上腺皮质激素释放因子（CRF）对可塑性的光学记录的神经元活动在胶质层（板II）的12-18天的大鼠脊髓切片染色的电压敏感染料。单脉冲测试刺激背根，激活A和C纤维诱发延长（>100毫秒）的光吸收的变化，在板层II。该响应表示沿切片深度沿着的所有元件的总膜电位变化。调理后高频刺激A纤维激活强度。测试刺激引起较少的神经元活性[在调节后45-60分钟时为-27 +/-1%（7），（平均+/- SE（n）），P < 0.01（*）]。在条件反射过程中应用CRF（1 μM，10 min），神经元活动被促进而不是抑制[+20 ± 3%（5），P < 0.05]。CRF单药治疗的影响不显著[-5 +/- 1%（4），P = 0.2]。在抑制性氨基酸拮抗剂荷包牡丹碱（1 μ M）和士的宁存在下，（0.3 μ M），相反，条件作用促进了这种促进作用[+27 +/-1%（12）*]，条件作用期间CRF处理剂量依赖性地抑制了这种促进作用[0.1 μ M：+18 +/-2%（5）*，1 μ M：+13 +/-1%（7）*]。尽管神经元间的活动可能有作用。这些结果表明，CRF可能对依赖于细胞条件的板层II内的兴奋性突触传递具有双重作用：它从长时程抑制的诱导转化为长时程增强（LTP），以及抑制LTP诱导。由于LTP被认为至少部分负责持续性疼痛，CRF可以调节诱导。(C)2003年国际疼痛研究协会。Elsevier B. V.出版，保留所有权利。
We examined the effects of corticotropin-releasing factor (CRF) on plasticity of optically recorded neuronal activity in the substantia gelatinosa (lamina II) of 12-18-day-old rat spinal cord slices stained with a voltage-sensitive dye. Single-pulse test stimulation to the dorsal root that activated A and C fibres evoked prolonged (>100 ms) light-absorption change in the lamina II. This response represents the gross membrane potential change of all elements along the slice depth. After conditioning high-frequency Stimulation of A-fibre-activating strength. test Stimulus elicited less neuronal activity [-27 +/- 1% (7), (average +/- SE (n)), P < 0.01 (*) at 45-60 min after conditioning]. When CRF (1 μM, 10 min) was applied during conditioning the neuronal activity was facilitated rather than suppressed [+20 ± 3% (5), P < 0.05]. CRF alone exhibited insignificant effect [-5 +/- 1% (4), P = 0.2]. In the presence of the inhibitory amino acid antagonists bicuculline (1 muM) and strychnine (0.3 muM) in the perfusate, in contrast, the conditioning facilitated it [+27 +/- 1% (12)*], and CRF treatment during conditioning inhibited the facilitation dose-dependently [0.1 muM: + 18 +/- 2% (5)*, 1 muM: + 13 +/- 1% (7)*]. Although interneuronal actions might contribute. these results suggest that CRF may have dual effects on excitatory synaptic transmission within the lamina II depending upon cellular conditions: it conversion from the induction of long-term depression to long-term potentiation (LTP), and inhibition of LTP induction. Since the LTP is thought to be responsible at least in part for the persistent pain, CRF could regulate the induction. (C) 2003 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.