Basal serum levels of FSH and estradiol in ovulatory and anovulatory women undergoing treatment by in-vitro maturation of immature oocytes.
Basal serum levels of FSH and estradiol in ovulatory and anovulatory women undergoing treatment by in-vitro maturation of immature oocytes.
复制标题
接受未成熟卵母细胞体外成熟治疗的排卵和无排卵女性的 FSH 和雌二醇基础血清水平。
作者：
T. Child;C. Sylvestre;I. Pirwany;Seang Tan
背景
这项研究的目的是确定基础血清FSH和雌二醇水平是否可以预测接受未成熟卵母细胞体外成熟(IVM)治疗的妇女的结局。
方法
数据来自123个未刺激的IVM周期。于周期第2~4天采集血清进行分析。患者在未成熟卵恢复前36h注射10000IU的人绒毛膜促性腺激素，恢复周期为9~14天。成熟卵母细胞行体外受精，2~3天后行胚胎移植。观察指标包括取回的未成熟卵母细胞数、卵母细胞成熟率、受精率、卵裂率和妊娠率。
结果
每个患者平均(范围)8(0-36)个未成熟卵母细胞。卵母细胞成熟率83%，受精率76%，卵裂率93%，妊娠率17.9%。血清FSH水平和多囊卵巢的存在是获取未成熟卵母细胞数量的显著独立预测因素，而患者年龄和基础雌二醇水平则不是。基础血清雌二醇水平与妊娠率显著相关(26%与11%)。基础血清雌二醇水平与妊娠率相关(P=0.032)。
结论
基础血清FSH和雌二醇水平的测定有助于预测未成熟卵母细胞的取回率和非刺激性体外受精治疗妇女的妊娠率。
BACKGROUND
The study aim was to establish whether basal serum levels of FSH and estradiol are predictive of outcome in women undergoing treatment by in-vitro maturation (IVM) of immature oocytes.
METHODS
Data were obtained from 123 unstimulated IVM cycles. Serum was taken between cycle days 2-4 for analysis. Patients received 10 000 IU of HCG 36 h before immature oocyte recovery that was performed between cycle days 9-14. IVM was performed and mature oocytes fertilized by ICSI, followed 2-3 days later by embryo transfer. Outcome measures included the number of immature oocytes retrieved, and the rates of oocyte maturation, fertilization, cleavage and pregnancy.
RESULTS
A median (range) of 8 (0-36) immature oocytes was retrieved per patient. Oocyte maturation, fertilization, cleavage and pregnancy rates were 83, 76, 93 and 17.9% respectively. Serum FSH levels and the presence of polycystic ovary were significant independent predictors of the number of immature oocytes retrieved, whilst patient age and basal estradiol level were not. A basal serum estradiol level >100 pmol/l was associated with a significantly higher pregnancy rate (26 versus 11% for estradiol <100 pmol/l; P = 0.032).
CONCLUSIONS
Measurement of basal serum levels of FSH and estradiol are useful in predicting the number of immature oocytes retrieved and the pregnancy rate in women undergoing unstimulated IVM treatment.