Bovine and Caprine Brucellosis in Bangladesh: Bayesian evaluation of four serological tests, true prevalence, and associated risk factors in household animals
Bovine and Caprine Brucellosis in Bangladesh: Bayesian evaluation of four serological tests, true prevalence, and associated risk factors in household animals
复制标题
DOI：
10.1007/s11250-016-1151-1
复制
发表时间：
2017-01-01
影响因子：
1.7
通讯作者：
Berkvens, Dirk
中科院分区：
文献类型：
--
作者：
Ahasan, Md. Shamim;Rahman, Md. Siddiqur;Berkvens, Dirk
进行了一项横断面研究，以估计布鲁氏菌的真实患病率。并确定与孟加拉国迪纳杰布尔和迈门辛地区布鲁氏菌病相关的相关风险因素/指标。共收集了320份分层随机血样，并使用虎红（RBT）、慢凝集（SAT）和间接竞争ELISA平行检测布鲁氏菌抗体。此外，还向每个家庭畜群所有者进行了结构化问卷调查，以收集有关潜在风险因素的信息。单变量和多变量logistic回归分析用于确定动物水平的潜在风险因素或指标。使用贝叶斯方法估计布鲁氏菌病的真实患病率沿着试验性能（Se和Sp）。牛和山羊的动物水平真实流行率估计值分别为9.70%（95%CPI 5.0- 16%）和6.3%（95%CPI 2.8- 11.0%）。SAT的灵敏度最高，范围为69.6%至78.9%，与其他试验相比，iELISA的特异性更高（97.4%至98.8%）。另一方面，与人工授精的牛相比，在自然繁殖的牛中发现了显著水平（P < 0.05）的布鲁氏菌血清阳性。在山羊中，与本地品种相比，外来品种与布鲁氏菌血清阳性率显著相关（P < 0.05）。有流产史和/或胎衣不下史的山羊也有显著性差异（P < 0.05）。具有先前流产记录的奶牛显示出较高的血清阳性几率（18倍）。评价的试验均不推荐单独用于牛和山羊布鲁氏菌病的诊断。
A cross-sectional study was carried out to estimate the true prevalence of Brucella spp. and identify allied risk factors/indicators associated with brucellosis in the Dinajpur and Mymensingh districts of Bangladesh. A total 320 stratified random blood samples were collected and tested in parallel for Brucella antibodies using Rose Bengal (RBT), slow agglutination (SAT), and indirect and competitive ELISA. In addition, a structured questionnaire was administered to each household herd owner to gather information regarding potential risk factors. Both univariate and multivariate logistic regression analyses were used to identify potential risk factors or indicators at animal level. A Bayesian approach was used to estimate the true prevalence of brucellosis along with the test performances (Se and Sp). The estimated animal level true prevalence in cattle was 9.70 % (95 % CPI 5.0-16 %) and in goat 6.3 % (95 % CPI 2.8-11.0 %). The highest sensitivity was achieved by SAT ranges from 69.6 to 78.9 %, and iELISA was found to be more specific (97.4 to 98.8 %) in comparison with other tests. On the other hand, a significant level of (P < 0.05) Brucella seropositivity was found in cattle that breed naturally compared with those that undergo artificial insemination. In goats, exotic breeds were significantly associated (P < 0.05) with Brucella seroprevalence compared with indigenous breeds. Goats with a previous records of abortion and/or retained placenta were also found to have significant levels (P < 0.05). Cows with previous abortion records showed higher odds (18 times) of being seropositive. None of the evaluated tests can be recommended to apply alone for the diagnosis of bovine and caprine brucellosis.