Integration of technologies for hepatic tissue engineering
Integration of technologies for hepatic tissue engineering
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DOI：
10.1007/10_029
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Yarmush, Martin L.
中科院分区：
文献类型：
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作者：
Nahmias, Yaakov;Berthiaume, Francois;Yarmush, Martin L.
肝脏是人体最大的内部器官，负责500多种代谢，调节和免疫功能。肝功能丧失导致肝功能衰竭，在美国每年导致超过25000例死亡。肝组织工程领域的努力包括设计生物人工肝系统以延长肝衰竭期间患者的生命，用于药物毒性筛选以及用于肝再生、缺血/再灌注损伤、纤维化、病毒感染和炎症的研究。本章将概述当前最先进的肝病学，包括离体灌注肝，肝切片和组织外植体的培养，胶原蛋白“三明治”和球状体上的肝细胞培养，肝细胞与非实质细胞的共培养，以及这些培养技术与微流体和反应器设计的整合。这项工作将讨论肝细胞培养中氧和培养基成分的作用，并提出有前途的肝细胞增殖和功能的新技术。我们还将讨论肝脏的发育、结构和功能，因为它们与这些培养技术有关。最后，我们将回顾当前的机遇和主要挑战，在整合细胞培养，生物反应器设计，和微技术开发新的应用系统。
The liver is the largest internal organ in the body, responsible for over 500 metabolic, regulatory, and immune functions. Loss of liver function leads to liver failure which causes over 25 000 deaths/year in the United States. Efforts in the field of hepatic tissue engineering include the design of bioartificial liver systems to prolong patient's lives during liver failure, for drug toxicity screening and for the study of liver regeneration, ischemia/reperfusion injury, fibrosis, viral infection, and inflammation. This chapter will overview the current state-of-the-art in hepatology including isolated perfused liver, culture of liver slices and tissue explants, hepatocyte culture on collagen "sandwich" and spheroids, coculture of hepatocytes with non-parenchymal cells, and the integration of these culture techniques with microfluidics and reactor design. This work will discuss the role of oxygen and medium composition in hepatocyte culture and present promising new technologies for hepatocyte proliferation and function. We will also discuss liver development, architecture, and function as they relate to these culture techniques. Finally, we will review current opportunities and major challenges in integrating cell culture, bioreactor design, and microtechnology to develop new systems for novel applications.