The K-segments of wheat dehydrin WZY2 are essential for its protective functions under temperature stress.
The K-segments of wheat dehydrin WZY2 are essential for its protective functions under temperature stress.
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小麦脱水蛋白 WZY2 的 K 片段对于其在温度胁迫下的保护功能至关重要
DOI：
10.3389/fpls.2015.00406
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发表时间：
2015
影响因子：
5.6
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Yang W;Zhang L;Lv H;Li H;Zhang Y;Xu Y;Yu J
脱水蛋白（DHN）是胚胎发育晚期丰富（莱亚）蛋白的第二组，在大多数植物中在冷、干旱、热或盐胁迫下表达上调。所有DHN都含有至少一个K片段，据信其通过形成两亲性螺旋在DHN功能中起重要作用。在前期的研究中，我们在低温和干旱胁迫条件下从普通小麦郑引1号中分离到了一个YSK 2型DHN基因wzy 2。构建了四种WZY 2截短衍生物，以敲除可能影响蛋白质功能的K-、Y-或S-片段。大肠杆菌活力增强，体外乳酸脱氢酶（LDH）活性保护和离体蛋白质聚集预防试验的体内试验表明，WZY 2作为一种保护剂，提高了温度变化过程中的胁迫耐受性。结果还表明，与不含K片段的截短衍生物不同，含有两个K片段的衍生物具有与全长WZY 2相似的显著效果，表明K片段是WZY 2的主要功能组分。此外，与其他片段相比，第一个K片段可能是WZY 2功能的最关键贡献者。总的来说，这项工作突出了DHN在离体缓解冷应激中的行为以及K段对DHN功能的贡献。
Dehydrins (DHNs), group 2 of late embryogenesis abundant (LEA) proteins, are up-regulated in most plants during cold, drought, heat, or salinity stress. All DHNs contain at least one K-segment, which is believed to play a significant role in DHN function by forming an amphipathic helix. In previous studies, wzy2, an YSK2-type DHN gene, was isolated from the Zhengyin 1 cultivar of Triticum aestivum under cold and drought stress treatment conditions. Four WZY2 truncated derivatives were constructed to knock out the K-, Y- or S-segment, which potentially affect the function of the protein. In vivo assays of Escherichia coli viability enhancement, in vitro lactate dehydrogenase (LDH) activity protection and ex vivo protein aggregation prevention assays revealed that WZY2 acted as a protectant and improved stress tolerance during temperature variation. The results also showed that unlike the truncated derivative without K-segments, the derivative containing two K-segments had remarkable effects that were similar to those of full-length WZY2, indicating that the K-segment is the major functional component of WZY2. Moreover, compared with the other segments, the first K-segment might be the most critical contributor to WZY2 functionality. In general, this work highlights the behavior of DHNs in relieving cold stress ex vivo and the contribution of the K-segment to DHN function.
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