Enhancement of polychlorinated biphenyl biodegradation by resuscitation promoting factor (Rpf) and Rpf-responsive bacterial community
Enhancement of polychlorinated biphenyl biodegradation by resuscitation promoting factor (Rpf) and Rpf-responsive bacterial community
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复苏促进因子 (Rpf) 和 Rpf 响应细菌群落增强多氯联苯生物降解
DOI：
10.1016/j.chemosphere.2020.128283
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发表时间：
2020
期刊：
影响因子：
8.8
通讯作者：
Sun Faqian
中科院分区：
文献类型：
--
作者：
Su Xiaomei;Li Si;Xie Mengqi;Tao Linqin;Zhou Yan;Xiao Yeyuan;Lin Hongjun;Chen Jianrong;Sun Faqian
多氯联苯（PCB）污染环境中本地细菌群落的活动与生物修复过程的效率密切相关。使用来自藤黄微球菌的复苏促进因子 (Rpf) 是一种在应激条件下复苏和刺激功能性细菌群体的有前途的方法。本研究旨在利用 Rpf 加速 Aroclor 1242 的生物降解，并探索通过添加 Rpf 复活的假定 PCB 降解剂。分别使用依赖于培养物和不依赖于培养物的方法研究了 Rpf 反应性细菌群体。结果证实，Rpf 能够增强 PCB 污染土壤中富集培养物的 PCB 降解，并提高对 PCB 耐受性较低的培养物的活性。高通量 16S rRNA 分析表明，Rpf 极大地改变了变形菌门中细菌种群的组成和丰度。复苏菌株的鉴定进一步表明，Rpf 响应群体主要以鞘氨醇单胞菌和假单胞菌为代表，它们很可能是增强 PCB 生物降解的关键 PCB 降解菌。
The activities of indigenous bacterial communities in polychlorinated biphenyls (PCBs) contaminated environments is closely related to the efficiency of bioremediation processes. Using resuscitation promoting factor (Rpf) fromMicrococcus luteusis a promising method for resuscitation and stimulation of functional bacterial populations under stressful conditions. This study aims to use the Rpf to accelerate the biodegradation of Aroclor 1242, and explore putative PCB degraders which were resuscitated by Rpf addition. The Rpf-responsive bacterial populations were investigated using culture-dependent and culture-independent approaches, respectively. The results confirm that Rpf was capable of enhancing PCB degradation of enriched cultures from PCB-contaminated soils, and improving the activities of cultures with low tolerance to PCBs. High-throughput 16S rRNA analysis displays that the Rpf greatly altered the composition and abundance of bacterial populations in the phylumProteobacteria. Identification of the resuscitated strains further suggests that the Rpf-responsive population was mostly represented bySphingomonasandPseudomonas, which are most likely the key PCB-degraders for enhanced biodegradation of PCBs.