Multiplex Reverse Transcriptase PCR Assay for Simultaneous Detection of Three Fish Viruses
Multiplex Reverse Transcriptase PCR Assay for Simultaneous Detection of Three Fish Viruses
复制标题
同时检测三种鱼类病毒的多重逆转录酶 PCR 检测
DOI：
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发表时间：
1999
影响因子：
9.4
通讯作者：
Bruce L. Nicholson
中科院分区：
文献类型：
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作者：
K. Williams;S. Blake;A. Sweeney;John T. Singer;Bruce L. Nicholson
建立了一种同时检测传染性胰腺坏死病毒(IPNV)、传染性造血坏死病毒(IHNV)和病毒性出血性败血症病毒(VHSV)的多重逆转录聚合酶链式反应(RT-PCR)方法。多重RT-PCR检测IPNV、IHNV和VHSV的敏感度分别为100、1和32.50%组织培养感染剂量/ml。
ABSTRACT A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.