Expression of the Prevotella loescheii adhesin gene (plaA) is mediated by a programmed frameshifting hop.
Expression of the Prevotella loescheii adhesin gene (plaA) is mediated by a programmed frameshifting hop.
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普氏菌粘附素基因 (plaA) 的表达是由程序性移码跳跃介导的。
DOI：
10.1128/jb.176.7.1944-1948.1994
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发表时间：
1994
影响因子：
3.2
通讯作者：
London,J
中科院分区：
文献类型：
--
作者：
Manch-Citron,JN;London,J
2.4 kb的plaA基因编码一个洛氏普雷沃菌半乳糖苷特异性粘附素，包含一个程序化的移码跳。移码区由两个UAA终止密码子、两个终止子之间的四个相同碱基的重复序列和一个茎环结构组成，该茎环结构具有形成位于第二个UAA下游的假结的潜力。茎环和假结是在许多逆转录病毒中发现的特征，其中移码更常见。在洛氏疟原虫plaA基因和mRNA转录物中鉴定了终止子、序列重复和二级结构。lacZ基因的密码子9和10之间的整个plaA移码区的框内融合允许在大肠杆菌中相对有效地表达β-半乳糖苷酶（对照的4至25%）。
The 2.4-kb plaA gene, which encodes a Prevotella loescheii galactoside-specific adhesin, contains a programmed frameshifting hop. The frameshift region consists of two UAA termination codons, two repeats of four identical bases between the terminators, and a stem-loop structure that has the potential to form a pseudoknot located downstream from the second UAA. The stem-loop and pseudoknot are features found in a number of retroviruses where frameshifting is a more common occurrence. The terminators, sequence repeats, and secondary structures were identified in both the P. loescheii plaA gene and the mRNA transcript. An in-frame fusion of the entire plaA frameshift region between codons 9 and 10 of the lacZ gene permitted relatively efficient expression (4 to 25% of that of the control) of beta-galactosidase in Escherichia coli.