Knockdown of lncRNA-PANDAR suppresses the proliferation, cell cycle and promotes apoptosis in thyroid cancer cells.
Knockdown of lncRNA-PANDAR suppresses the proliferation, cell cycle and promotes apoptosis in thyroid cancer cells.
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DOI：
10.17179/excli2017-113
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发表时间：
2017
期刊：
影响因子：
4.6
通讯作者：
Luo D
中科院分区：
文献类型：
--
作者：
Li Z;Gao B;Hao S;Tian W;Chen Y;Wang L;Zhang X;Luo D
已发现长链非编码RNA（lncRNA）在多种人类癌症中显示出重要的调节作用。Lnc-RNA PANDAR是一种新发现的lncRNA，在多种肿瘤中有异常表达。然而，其在甲状腺癌中的表达和生物学功能知之甚少。本研究采用实时荧光定量PCR（qRT-PCR）方法检测64例甲状腺癌组织中PANDAR的表达。我们发现PANDAR在甲状腺癌组织中的表达较癌旁组织上调。体外功能实验表明，PANDAR基因的敲低可以抑制甲状腺癌细胞的增殖、细胞周期进程、诱导凋亡、抑制侵袭。因此，我们的研究提供了证据表明PANDAR可能作为甲状腺癌患者治疗的潜在靶点。
Long non-coding RNAs (lncRNAs) have been found to show important regulatory roles in various human cancers. Lnc-RNA PANDAR is a novel identified lncRNA that was previously reported to show abnormal expression pattern in various cancers. However, little is known of its expression and biological function in thyroid cancer. Here, we used the quantitative real-time PCR (qRT-PCR) to determine the expression of PANDAR in 64 thyroid cancer tissues. We found that expression of PANDAR was up-regulated in thyroid cancer tissues compared with adjacent non-tumor tissues. Functional assays in vitro demonstrated that knockdown of PANDAR could inhibit proliferation, cell cycle progression, induces the apoptosis, inhibit invasion of thyroid cancer cells. Thus, our study provides evidence that PANDAR may function as a potential target for treatment for patients with thyroid cancer.