Effect of hyperthermia on selective expression of HL-60 heat shock proteins.
Effect of hyperthermia on selective expression of HL-60 heat shock proteins.
复制标题
高温对 HL-60 热休克蛋白选择性表达的影响。
DOI：
10.1007/bf02985156
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
North,JA
中科院分区：
文献类型：
--
作者：
Burns,CP;Wagner,BA;North,JA
热疗法被实验性地用于治疗人类恶性肿瘤。研究了热传递速率和热耐受性对人HL-60细胞分化前后热休克蛋白（hsp）表达的影响。这种白血病细胞合成多种热休克蛋白以应对高温。最明显和一致的蛋白质是在高度保守的应激诱导的多肽hsp 70家族内，其解析为hsp 69/72双联体。通过逐渐加热使细胞产生耐热性，选择性地不能表达hsp 70双联体，即使合成了其他的hsp。成熟的HL-60细胞诱导分化的视黄酸孵育时表达的热休克蛋白的充分补充时，暴露于立即热，但有选择性删除热休克蛋白70逐渐高温。这一热休克蛋白选择性诱导模型证实了热休克蛋白的合成与热耐受性在HL-60细胞中是分离的。提示热休克蛋白70在白血病细胞的热耐受性中不起强制性作用。
Hyperthermia is used experimentally to treat human malignancy. The effect of heat delivery rate and thermotolerance on the expression of heat shock proteins (hsp) by the human HL-60 cell line before and after differentiation was studied. This leukemia cell synthesized multiple hsp in response to elevated temperatures. The most obvious and consistent proteins were within the highly conserved stress-inducible family of polypeptides hsp70 which resolved as a hsp69/72 doublet. Cells which were made thermotolerant by gradual heating selectively failed to express the hsp70 doublet even though other hsp were synthesized. Mature HL-60 cells induced to differentiate by incubation in retinoic acid expressed a full complement of hsp when exposed to immediate heat, but there was selective deletion of hsp70 with gradual hyperthermia. This model for selective induction of hsp confirms that synthesis of hsp and thermotolerance can be dissociated in the HL-60. It suggests that the hsp70 does not play an obligatory role in thermotolerance of this human leukemia cell.