Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development
Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development
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DOI：
10.7554/elife.24336
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发表时间：
2017-04-19
期刊：
影响因子：
7.7
通讯作者：
Oecking, Claudia
中科院分区：
文献类型：
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作者：
Keicher, Jutta;Jaspert, Nina;Oecking, Claudia
真核生物14-3-3蛋白通过磷酸化依赖的蛋白质-蛋白质相互作用参与多种生物学过程的调节。拟南芥基因组编码两组14-3-3s，其中一组14 -3- 3s被认为履行保守的细胞功能。在这里，我们评估了祖先14-3-3 β组成员的体内作用。在幼苗中通过RNA干扰和人工microRNA对它们的同时和有条件的抑制导致植物激素生长素和相关的生长素运输相关表型的分布模式改变，例如无重力生长。此外，需要14-3-3个成员的PIN形成的生长素外排载体在质膜内的显着极性分布。在定义后高尔基体贩运过程中的缺陷被证明是这种表型的原因，可能是由于缺乏直接的14-3-3与膜贩运的关键因素的相互作用。综上所述，我们的数据表明，古老的14-3-3-3-NH 4基团成员在调节PIN极性和植物发育中起着重要作用。
Eukaryotic 14-3-3 proteins have been implicated in the regulation of diverse biological processes by phosphorylation-dependent protein-protein interactions. The Arabidopsis genome encodes two groups of 14-3-3s, one of which epsilon is thought to fulfill conserved cellular functions. Here, we assessed the in vivo role of the ancestral 14-3-3 epsilon group members. Their simultaneous and conditional repression by RNA interference and artificial microRNA in seedlings led to altered distribution patterns of the phytohormone auxin and associated auxin transport related phenotypes, such as agravitropic growth. Moreover, 14-3-3 epsilon members were required for pronounced polar distribution of PIN-FORMED auxin efflux carriers within the plasma membrane. Defects in defined post-Golgi trafficking processes proved causal for this phenotype and might be due to lack of direct 14-3-3 interactions with factors crucial for membrane trafficking. Taken together, our data demonstrate a fundamental role for the ancient 14-3-3 epsilon group members in regulating PIN polarity and plant development.