Single cell transcriptome research in human placenta.
Single cell transcriptome research in human placenta.
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DOI：
10.1530/rep-20-0231
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发表时间：
2020-12
期刊：
影响因子：
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通讯作者：
Garmire LX
中科院分区：
文献类型：
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作者：
Li H;Huang Q;Liu Y;Garmire LX
人类胎盘是一个复杂的异质性器官，连接母亲和胎儿，支持胎儿发育。胎盘结构成分的改变与各种妊娠并发症有关。为了揭示正常和患病胎盘中各种胎盘细胞类型之间的异质性，以及阐明胎盘细胞群体内的分子相互作用，在过去几年中，一种称为单细胞RNA-seq（或scRNA-seq）的新基因组学技术已经被采用。本文综述了scRNA-seq技术的原理，并总结了近年来scRNA-seq技术在不同胎龄和妊娠并发症（如早产和先兆子痫）中对人类胎盘的研究。我们列出了可供公众使用的计算分析平台和资源。最后，我们讨论了胎盘单细胞研究的未来感兴趣的领域，以及完成这些研究所需的数据分析。
Human placenta is a complex and heterogeneous organ interfacing between the mother and the fetus that supports fetal development. Alterations to placental structural components are associated with various pregnancy complications. To reveal the heterogeneity among various placenta cell types in normal and diseased placentas, as well as elucidate molecular interactions within a population of placental cells, a new genomics technology called single cell RNA-seq (or scRNA-seq) has been employed in the last couple of years. Here we review the principles of scRNA-seq technology, and summarize the recent human placenta studies at scRNA-seq level across gestational ages as well as in pregnancy complications, such as preterm birth and preeclampsia. We list the computational analysis platforms and resources available for the public use. Lastly, we discuss the future areas of interest for placenta single cell studies, as well as the data analytics needed to accomplish them.