F-type proton-pumping ATPase mediates acid tolerance in <i>Streptococcus mutans</i>
F-type proton-pumping ATPase mediates acid tolerance in <i>Streptococcus mutans</i>
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F 型质子泵 ATP 酶介导变形链球菌的酸耐受性
DOI：
10.1093/jambio/lxad073
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发表时间：
2023
影响因子：
4
通讯作者：
Nakanishi-Matsui Mayumi
中科院分区：
文献类型：
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作者：
Sekiya Mizuki;Ikeda Kazuya;Yonai Ayaka;Ishikawa Taichi;Shimoyama Yu;Kodama Yoshitoyo;Sasaki Minoru;Nakanishi-Matsui Mayumi
目的变形链球菌在酸性条件下对质子泵 F 型 ATP 酶 (F-ATP 酶) 抑制剂高度敏感。在这里，我们研究了 S 的作用。 mutansF-ATPase 使用表达 F-ATPaseβ 亚基的细菌进行耐酸，其水平低于野生型菌株。方法和结果我们产生了突变体 S。 mutans 表达 F-ATP 酶催化 β 亚基的水平低于野生型细菌。突变细胞在pH 5.30时表现出明显较慢的生长速率，而在pH 7.40时与野生型细胞的生长速率基本相同。此外，突变体的集落形成能力在pH<4.30时降低，但在pH 7.40时不降低。因此，S的增长率和生存率。结论结合我们之前的观察，本研究表明F-ATPase参与了S的耐酸机制。突变体通过从细胞质分泌质子。
AimsStreptococcus mutansis highly sensitive to inhibitors of proton-pumping F-type ATPase (F-ATPase) under acidic conditions. Herein, we investigated the role ofS. mutansF-ATPase in acid tolerance using a bacterium expressing the F-ATPaseβsubunit at lower levels than the wild-type strain.Methods and resultsWe generated a mutantS. mutansexpressing the catalytic β subunit of F-ATPase at lower levels than the wild-type bacterium. The mutant cells exhibited a significantly slower growth rate at pH 5.30, whereas the rate was essentially the same as that of wild-type cells at pH 7.40. In addition, the colony-forming ability of the mutant was decreased at pH <4.30 but not at pH 7.40. Thus, the growth rate and survival ofS. mutansexpressing low levels of the β subunit were reduced under acidic conditions.ConclusionsTogether with our previous observations, this study indicates that F-ATPase is involved in the acid tolerance mechanism ofS. mutansby secreting protons from the cytoplasm.