In vitro susceptibility of Achromobacter spp. isolates: comparison of disk diffusion, Etest and agar dilution methods
In vitro susceptibility of Achromobacter spp. isolates: comparison of disk diffusion, Etest and agar dilution methods
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DOI：
10.1016/j.ijantimicag.2009.08.015
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发表时间：
2010-01-01
影响因子：
10.8
通讯作者：
Vay, Carlos
中科院分区：
文献类型：
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作者：
Almuzara, Marisa;Limansky, Adriana;Vay, Carlos
本研究分析了临床分离的92株无色杆菌对18种抗菌药物的敏感性。将纸片扩散法和eest法与琼脂稀释法进行比较，确定纸片扩散法对被试抗菌素的药敏和耐药断点。最有效的抗生素是哌拉西林、哌拉西林/他唑巴坦和碳青霉烯类。采用线性最小二乘回归方法，建立亚胺培南、美罗培南、厄他培南、甲氧苄啶/磺胺甲恶唑（SXT）等抗菌药物的断点。其他有效抗生素，如哌拉西林和米诺环素，可以用Etest方法进行检测。庆大霉素、强力霉素、四环素等活性较弱的抗生素可采用纸片扩散法检测。对于测试的其余抗菌药物，由于误差百分比高和/或获得的线性回归系数差，无法建立断点。因此，这些抗菌药物应通过最低抑菌浓度测定进行测试。综上所述，我们建议对耐药和易感患者分别使用以下区域直径断点：亚胺培南= 22 mm；美罗培南= 24 mm；厄他培南= 24 mm；庆大霉素= 21 mm；SXT = 28 mm；四环素= 29 mm；强力霉素= 24 mm。(C) 2009 Elsevier B.V.与International Society of Chemotherapy。版权所有。
In this study, we analysed the antimicrobial susceptibility of 92 strains of Achromobacter spp. isolated from clinical samples to 18 antimicrobial agents. The disk diffusion method and Etest were compared with the agar dilution method, and the breakpoints of susceptibility and resistance for the disk diffusion method for the antimicrobials tested were determined. The most active antibiotics were piperacillin, piperacillin/tazobactam and the carbapenems. By applying the linear least-squares regression method, breakpoints could be established for antibiotics active against this genus such as imipenem, meropenem, ertapenem and trimethoprim/sulfamethoxazole (SXT). Other active antibiotics, such as piperacillin and minocycline, could be tested by the Etest method. The less active antibiotics such as gentamicin, doxycycline and tetracycline could be tested by the disk diffusion method. For the rest of the antimicrobial agents tested, breakpoints could not be established owing to the high percentage of errors and/or the poor linear regression coefficient obtained. Therefore, these antimicrobial agents should be tested by minimal inhibitory concentration determination. In summary, we recommend the following zone diameter breakpoints for resistant and susceptible, respectively: = 22 mm for imipenem; = 24 mm for meropenem; = 24 mm for ertapenem; = 21 mm for gentamicin; = 28 mm for SXT; = 29 mm for tetracycline; and = 24 mm for doxycycline. (C) 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.