Folate deficiency in rats fed diets containing free amino acids or intact proteins.
Folate deficiency in rats fed diets containing free amino acids or intact proteins.
复制标题
DOI：
10.1093/jn/118.9.1089
复制
发表时间：
1988-09
期刊：
影响因子：
--
通讯作者：
Rosemary L. Walzem;Andrew J. Clifford
中科院分区：
文献类型：
--
作者：
Rosemary L. Walzem;Andrew J. Clifford
Development of folate deficiency (FD) was evaluated in weanling rats fed diets containing mixtures of free amino acids or of vitamin-free casein and gelatin as sources of dietary nitrogen. FD could be produced in 21 d with amino acid diets that promoted maximum growth rate, were completely devoid of folate and contained 1% succinylsulfathiazole. Growth retardation and blood dyscrasia associated with FD could not be demonstrated in rats fed diets containing casein and gelatin as nitrogen sources because the vitamin-free casein contained low but measurable levels of folate. The most effective protocol to produce experimental FD in rats is to feed a folate-free diet that otherwise supports maximum growth in young animals. Additional modifications such as use of methotrexate or amino acid-imbalanced or protein-deficient diets are unnecessary.
