Quantitative trait loci controlling aluminium tolerance in two accessions of Arabidopsis thaliana (Landsberg erecta and Cape Verde Islands)
Quantitative trait loci controlling aluminium tolerance in two accessions of Arabidopsis thaliana (Landsberg erecta and Cape Verde Islands)
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DOI：
10.1111/j.1365-3040.2005.01388.x
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发表时间：
2005-12-01
影响因子：
7.3
通讯作者：
Koyama, H
中科院分区：
文献类型：
--
作者：
Kobayashi, Y;Furuta, Y;Koyama, H
以拟南芥Ler/Cvi重组自交系为材料，对耐铝性进行了QTL分析。第5天的相对根长（RRL）（pH 5.0时含4 μ M Al的根长/不含Al的根长）被用作QTL分析的铝耐性指标。利用h h（B）（2）= 0.91的数据集，检测到2个QTL，分别位于第1染色体的顶部和第3染色体的底部。这些QTL分别解释了耐铝性表型变异的40%和16%，以及Cvi等位基因的正效应。第1染色体上的QTL与另一个重组自交群体中的主效QTL重叠，可能与苹果酸分泌有关。通过一个完整的成对搜索，发现了11组上位性互作位点对，这些位点对解释了RI群体间的超亲分离。几个上位相互作用共享相同的染色体区域，表明调节蛋白可能参与拟南芥耐铝。
Quantitative trait loci (QTL) analysis of aluminium (Al) tolerance was performed using Ler/Cvi recombinant inbred (RI) lines of Arabidopsis thaliana. Relative root length (RRL) (root length with 4 mu M Al/root length with no Al at pH 5.0) on day 5 was used as the Al tolerance index for QTL analysis. Al tolerance judged by RRL was well correlated to tolerance judged by other indexes, including accumulation of callose, reactive oxygen species in the root apex and growth performance on acid soil containing a large amount of exchangeable Al. Using data sets with an h h(b)(2) of 0.91, two QTLs were detected at the top of chromosome I and bottom of chromosome 3. These QTLs explained 40 and 16% of the phenotypic variation of Al tolerance, respectively, and the positive effect of the Cvi allele. The QTL on chromosome 1 overlapped with a major QTL in another recombinant inbred population, and is possibly related to malate excretion. A complete pair-wise search revealed 11 sets of epistatic interacting loci pairs, which accounted for the transgressive segregation among the RI population. Several epistatic interactions shared the same chromosomal region, indicating the possible involvement of regulatory proteins in Al tolerance in Arabidopsis.