Genetic changes accompanying increased fitness in evolving populations of Escherichia coli.
Genetic changes accompanying increased fitness in evolving populations of Escherichia coli.
复制标题
不断进化的大肠杆菌种群中伴随适应性增强的遗传变化。
DOI：
10.1093/genetics/130.2.241
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Adams,J
中科院分区：
文献类型：
--
作者：
Modi,RI;Castilla,LH;Puskas-Rozsa,S;Helling,RB;Adams,J
用含有质粒pBR322衍生物的单个克隆起始两个大肠杆菌群体，在葡萄糖限制的连续培养中维持了很长时间。在这两个种群中，经过大量的世代后，从质粒中分离出转座子Tn3已整合到细菌染色体中的克隆。在这两种情况下，在维持种群的环境中，转位显示出增加了大约6-7%的相对适合度。整合位点被定位到大约13.2 min（种群1）和大约32.8 min（种群2）。
Two populations of Escherichia coli, each initiated with a single clone containing a derivative of the plasmid pBR322, were maintained for long periods in glucose-limited continuous culture. In both populations, after an extensive number of generations had elapsed, clones were isolated in which the transposon Tn3 from the plasmid had integrated into the bacterial chromosome. In both cases examined, the transpositions were shown to increase relative fitness approximately 6-7%, in the environment in which the populations were maintained. The loci of integration were mapped to approximately 13.2 min (population 1) and approximately 32.8 min (population 2).