Effects of basic fibroblast growth factor on experimental diabetic neuropathy in rats
Effects of basic fibroblast growth factor on experimental diabetic neuropathy in rats
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DOI：
10.2337/db05-1160
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发表时间：
2006-05-01
期刊：
影响因子：
7.7
通讯作者：
Nakamura, J
中科院分区：
文献类型：
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作者：
Nakae, M;Kamiya, H;Nakamura, J
碱性成纤维细胞生长因子（bFGF）刺激血管生成并诱导神经细胞再生。我们研究了 bFGF 对链脲佐菌素诱导的糖尿病大鼠糖尿病神经病变的影响。按如下方式用人重组bFGF治疗糖尿病大鼠：1）静脉内给药，2）用交联明胶水凝胶（CGH）肌内注射到大腿和比目鱼肌中，以及3）用盐水肌内注射。 10天或30天后，测量坐骨胫神经和尾神经的运动神经传导速度（MNCV）、对机械刺激的敏感性、坐骨神经血流量（SNBF）和视网膜血流量（RBF）。糖尿病大鼠的坐骨胫骨和尾神经的 MNCV 延迟、痛觉减退和 SNBF 减少均在 10 天（但不是 30 天）静脉注射 bFGF 后得到改善。肌内注射 bFGF 和 CGH 也改善了 10 天和 30 天后的坐骨-胫骨 MNCV、痛觉减退和 SNBF，但尾部 MNCV 没有改善。然而，用生理盐水肌肉注射bFGF则没有明显效果。 bFGF 不会显着改变正常或糖尿病大鼠的 RBF。这些观察结果表明bFGF可能对糖尿病神经病变具有治疗价值，并且CGH作为bFGF的载体可以发挥重要作用。
Basic fibroblast growth factor (bFGF) stimulates angiogenesis and induces neural cell regeneration. We investigated the effects of bFGF on diabetic neuropathy in streptozotocin-induced diabetic rats. Diabetic rats were treated with human recombinant bFGF as follows: 1) intravenous administration, 2) intramuscular injection into thigh and soleus muscles with cross-linked gelatin hydrogel (CGH), and 3) intramuscular injection with saline. Ten or 30 days later, the motor nerve conduction velocity (MNCV) of the sciatic-tibial and caudal nerves, sensitivity to mechanical stimuli, sciatic nerve blood flow (SNBF), and retinal blood flow (RBF) were measured. Delayed MNCV in the sciatic-tibial and caudal nerves, hypoalgesia, and reduced SNBF in diabetic rats were all ameliorated by intravenous administration of bFGF after 10, but not 30, days. Intramuscular injection of bFGF with CGH also improved sciatic-tibial MNCV, hypoalgesia, and SNBF after 10 and 30 days, but caudal MNCV was not improved. However, intramuscular injection of bFGF with saline had no significant effects. bFGF did not significantly alter RBF in either normal or diabetic rats. These observations suggest that bFGF could have therapeutic value for diabetic neuropathy and that CGH could play important roles as a carrier of bFGF.