Component-resolved diagnosis of house-dust mite allergy with purified natural and recombinant mite allergens
Component-resolved diagnosis of house-dust mite allergy with purified natural and recombinant mite allergens
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DOI：
10.1111/j.1365-2222.2004.1930.x
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发表时间：
2004-04-01
影响因子：
6.1
通讯作者：
Valenta, R
中科院分区：
文献类型：
--
作者：
Pittner, G;Vrtala, S;Valenta, R
背景螨是最常见和最强的过敏原来源。如果过敏原避免不可能或不成功，则经常使用螨过敏原提取物进行免疫治疗。然而，在这方面，螨特异性免疫治疗的结果一直备受争议。目的本研究的目的是发展诊断概念，可能有助于改善Der p变应原提取物免疫治疗患者的选择，并可能用于接受这种治疗的患者的免疫监测。方法在中欧螨中测定Der p变应原提取物的IgE反应性。通过IgE免疫印迹法和使用一组7种纯化的天然或重组Der p变应原（nDer p 1、nDer p 4、rDer p 2、rDer p 5、rDer p 7、rDer p 8、rDer p 10）来检测过敏性群体。此外，我们调查的致敏性和交叉反应性屋尘和存储螨过敏原提取物的CAP FEIA测量和IgE竞争studies.Results超过95%的患者可以诊断与nDer p 1和rDer p 2的组合。通过所用的方法，我们可以区分主要对主要Der p过敏原致敏的螨过敏患者（Der p 1，Der p 2）来自具有广泛致敏特征的患者，包括高交叉反应性过敏原（例如，Der p 10：结论诊断试验含有主要的螨过敏原，（即Der p 1、Der p 2）和高交叉反应性螨变应原（例如Der p 10）的组合可以改善患者对Der p提取物免疫疗法的诊断选择。这些测试也可用于接受免疫治疗的患者的免疫监测。
Background Mites belong to the most frequent and potent allergen sources. Immunotherapy with mite allergen extracts is frequently performed if allergen avoidance is not possible or successful. However, highly controversial results have been reported for mite-specific immunotherapy.Objective The aim of this study was to develop diagnostic concepts that may contribute to an improved selection of patients for immunotherapy with Der p allergen extracts and that may be used for immunological monitoring of patients undergoing this treatment.Methods The IgE reactivity profiles to Der p extract were determined in a Middle European mite-allergic population by IgE immunoblotting and by using a panel of seven purified natural or recombinant Der p allergens (nDer p 1, nDer p 4, rDer p 2, rDer p 5, rDer p 7, rDer p 8, rDer p 10). Furthermore, we investigated the sensitization and cross-reactivity to house-dust- and storage-mite allergen extracts by CAP FEIA measurements and by IgE competition studies.Results More than 95% of the patients could be diagnosed with a combination of nDer p 1 and rDer p 2. With the methods used, we could discriminate mite-allergic patients who were mainly sensitized to the major Der p allergens (Der p 1, Der p 2) from patients with a broad sensitization profile, including highly cross-reactive allergens (e.g. Der p 10: tropomyosin) as well as reactivity to storage mites.Conclusions Diagnostic tests containing the major mite allergens (i.e. Der p 1, Der p 2) and highly cross-reactive mite allergens (e.g. Der p 10) may improve the diagnostic selection of patients for immunotherapy with Der p extracts. These tests may also be used for the immunological monitoring of patients undergoing immunotherapy.