Thrombospondin-4 and matrix three-dimensionality in axon outgrowth and adhesion in the developing retina
Thrombospondin-4 and matrix three-dimensionality in axon outgrowth and adhesion in the developing retina
复制标题
DOI：
10.1016/j.exer.2006.12.014
复制
发表时间：
2007-04-01
影响因子：
3.4
通讯作者：
Clegg, Dennis O.
中科院分区：
文献类型：
--
作者：
Dunkle, Erin Tolhurst;Zaucke, Frank;Clegg, Dennis O.
血小板反应蛋白-4（TSP-4）是细胞外基质的一种大的五聚体糖蛋白，被描述为促进神经突生长的分子。然而，TSP-4促进发育中的眼神经突生长的方式尚不清楚。在这里，我们表明TSP-4在发育的适当时间存在，并在发育中的小鼠视网膜内显示出与视网膜神经节细胞（RGC）神经突生长中的作用一致的定位模式。此外，结果表明，虽然TSP-4单独不支持粘附或神经突延伸，但它增强了层粘连蛋白促进胚胎视网膜细胞粘附和神经突生长的能力。增强的机制部分地基于TSP-4增强基质基质内层粘连蛋白的三维性和/或聚集的能力。这些结果支持TSP-4作为ECM中粘附和轴突生长促进分子的组织者以优化视网膜神经节细胞反应的模型。爱思唯尔有限公司出版
Thrombospondin-4 (TSP-4), a large pentameric glycoprotein of the extracellular matrix, has been described as a neurite outgrowth-promoting molecule. However, the means by which TSP-4 promotes neurite outgrowth in the developing eye is unclear. Here we show that TSP-4 is present at the appropriate time in development and displays a localization pattern within the developing mouse retina consistent with a role in retinal ganglion cell (RGC) neurite outgrowth. Furthermore, results indicate that while TSP-4 alone does not support adhesion or neurite extension, it enhances the ability of laminins to promote adhesion and neurite outgrowth of embryonic retinal cells. The mechanism of enhancement is, in part, based on the ability of TSP-4 to enhance the three-dimensionality and/or clustering of laminins within the substrate matrix. These results support a model where TSP-4 acts as an organizer of adhesive and axon outgrowth-promoting molecules in the ECM to optimize retinal ganglion cell responses. Published by Elsevier Ltd.