Label-free cell aggregate formation based on the magneto-Archimedes effect
Label-free cell aggregate formation based on the magneto-Archimedes effect
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DOI：
10.1063/1.3581883
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发表时间：
2011-04-18
影响因子：
4
通讯作者：
Morishima, Keisuke
中科院分区：
文献类型：
--
作者：
Akiyama, Yoshitake;Morishima, Keisuke
这封信演示了通过无标记三维（3D）细胞组装方法形成细胞聚集体，该方法基于磁阿基米德效应。在培养液中加入钆配合物以增强细胞的抗磁性，从而使无标记的磁性操作成为可能。证实钆络合物的细胞毒性足够低，暴露时间少于1天。用这种方法，细胞在约20分钟内聚集成蛋形，并在一天内变成球状体。这些结果表明，该方法是一种很有前途的方法来构建任意形状的三维组织。(C)2011年美国物理学会。[doi：10.1063/1.3581883]
This letter demonstrates formation of cell aggregates by a label-free three-dimensional (3D) cell assembly method, which is based on the magneto-Archimedes effect. Gadolinium complex was added to the culturing medium to enhance the diamagnetic property of the cells, by which label-free magnetic manipulation became possible. Cytotoxicity of the gadolinium complex was confirmed to be sufficiently low for exposure less than one day. With this method, the cells aggregated into an egg-shape in about 20 min and became a spheroid in one day. These results suggest that this method is a promising approach to construct arbitrarily-shaped 3D tissues. (C) 2011 American Institute of Physics. [doi:10.1063/1.3581883]