Constitutive production by the WEHI-3 cell line of B cell growth and differentiation factor that co-purifies with interleukin 1.
Constitutive production by the WEHI-3 cell line of B cell growth and differentiation factor that co-purifies with interleukin 1.
复制标题
WEHI-3 细胞系组成型生产 B 细胞生长和分化因子，与白细胞介素 1 共纯化。
作者：
R. Booth;R. Prestidge;J. Watson
骨髓单核细胞系 WEHI-3 组成型产生一种影响培养物中小鼠 B 细胞生长和分化的因子。该细胞系还分泌集落刺激因子 (CSF)、白细胞介素 1 (IL 1)，但不分泌白细胞介素 2。通过 AcA54 凝胶过滤、DEAE-Sephacel 离子交换层析和缓冲液电聚焦进行连续纯化，清楚地分离了 CSF 活性中的 B 细胞生长和分化因子 (BGDF)，但未能将 BGDF 与 IL 1 分离。然而，负责 BGDF/IL 1 活性的 WEHI-3 衍生材料表现出不同的活性。 DEAE 色谱法（175 mM NaCl 洗脱）的行为与 P388D1 细胞系 IL 1 报道的（50 mM NaCl 洗脱）的行为相同。在缺乏 T 细胞和贴壁细胞的正常脾细胞培养物中，WEHI-3 BGDF/IL 1 可以诱导 B 细胞生长和分化，但在 B 细胞缺陷的 CBA/N 小鼠的脾细胞培养物中则不然，即使来自此类小鼠的胸腺细胞对 IL 1 显示出正常反应。BGDF/IL 1 诱导的显着 B 细胞增殖仅在存在抗小鼠 IgM 抗体的致屈服浓度时才明显，但在这些条件下，很少产生抗体形成细胞（AFC）。相比之下，B 细胞分化为 AFC 的情况仅在该因子存在的情况下发生，并且这种反应被抗 IgM 抑制。因此，BGDF/IL 1 诱导的 B 细胞增殖和分化之间似乎存在相互关系。根据 BGDF 最近的其他研究讨论了这些结果的意义。
The myelomonocytic cell line WEHI-3 produces constitutively a factor that affects the growth and differentiation of murine B cells in culture. This cell line also secretes colony-stimulating factors (CSF), interleukin 1 (IL 1) but not interleukin 2. Sequential purification through AcA54 gel filtration, DEAE-Sephacel ion exchange chromatography, and buffer electrofocussing clearly resolved the B cell growth and differentiation factor (BGDF) from the CSF activities but failed to separate BGDF from IL 1. The WEHI-3-derived material responsible for BGDF/IL 1 activity, however, exhibited different behavior on DEAE chromatography (elution at 175 mM NaCl) to that reported for IL 1 from the P388D1 cell line (elution at 50 mM NaCl). B cell growth and differentiation could be induced by WEHI-3 BGDF/IL 1 in cultures of normal spleen cells depleted of T cells and adherent cells but not in cultures of spleen cells from B cell-deficient CBA/N mice, even though thymocytes from such mice displayed a normal response to IL 1. Significant B cell proliferation induced by BGDF/IL 1 was apparent only in the presence of submitogenic concentrations of anti-mouse IgM antibodies, but under these conditions few antibody-forming cells (AFC) were generated. In contrast, B cell differentiation to AFC occurred in the presence of the factor alone, and this response was inhibited by anti-IgM. Thus there appeared to be a reciprocal relationship between B cell proliferation and differentiation induced by BGDF/IL 1. The significance of these results is discussed in the light of other recent studies of BGDF.