Binding assistance triggering attachments of hairpin DNA onto gold nanoparticles.
Binding assistance triggering attachments of hairpin DNA onto gold nanoparticles.
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DOI：
10.1021/ac402908y
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发表时间：
2013-11
影响因子：
7.4
通讯作者：
Cheng Zhang;Jingjing Ma;Jing Yang;Shi Liu;Jin Xu
中科院分区：
文献类型：
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作者：
Cheng Zhang;Jingjing Ma;Jing Yang;Shi Liu;Jin Xu
在这里，我们提出了一种策略，触发thoilated发夹DNA到金纳米粒子的单价附件，辅助分子结合。如果不结合辅助链，由于空间障碍，很难控制巯基化发夹DNA的附着。通过引入结合辅助链，可以使硫醇基团紧密接近AuNP的表面，这将大大增加局部分子浓度和附着效率。在实验中，通过DNA链分支迁移和纳米金簇的动态组装方法对该策略进行了验证。此外，产生了具有明确定义的DNA支架排列的独特且复杂的AuNP簇。使用这种方法，它能够选择性地操纵和控制不同种类和数量的DNA附着在AuNPs上。我们的策略也可以扩展到组装大型复杂的DNA/AuNPs可编程结构和纳米器件。
Here, we present a strategy to trigger monovalent attachments of thoilated hairpin DNA onto AuNP, assisted by molecular binding. Without binding helper strands, it is hard to control the attachments of thiolated hairpin DNA, because of spatial hindrances. By introducing a binding helper strand, the thiol-group can be brought into close proximity to the surface of AuNPs, which will greatly increase the local molecular concentration and attaching efficiency. In the experiments, the strategy is verified by the methods of DNA strand branch migration and dynamic assembly of AuNPs clusters. In addition, unique and complex AuNPs clusters with well-defined arrangements of DNA scaffolds are produced. Using this method, it is able to selectively manipulate and control different kinds and numbers of DNA attaching onto AuNPs. Our strategy also could be extended to assembling large complicated DNA/AuNPs programmable structures and nanodevices.