Antibacterial agents and release of periplasmic pertussis toxin from Bordetella pertussis.
Antibacterial agents and release of periplasmic pertussis toxin from Bordetella pertussis.
复制标题
抗菌剂和百日咳博德特氏菌周质百日咳毒素的释放。
DOI：
10.1128/aac.44.5.1383-1386.2000
复制
发表时间：
2000
影响因子：
4.9
通讯作者：
Weiss,AA
中科院分区：
文献类型：
--
作者：
Craig-Mylius,KA;Weiss,AA
百日咳毒素在分泌前积聚在百日咳博德特氏菌的周质中，我们检查了其在用抗菌药物处理后的命运。抑制蛋白质合成（红霉素和氯霉素）、转录（利福平）或细胞壁生物合成（头孢哌酮和哌拉西林）的抗生素和硫酸镁（抑制百日咳毒素的转录，但不抑制细菌生长）都不能阻止预先形成的毒素的释放。相反，影响细菌膜的药物，如多粘菌素 B、利多卡因、普鲁卡因和乙醇，会抑制预先形成的百日咳毒素的释放。这些结果表明，百日咳毒素分泌不需要新的蛋白质合成，但需要功能性膜复合物。
Pertussis toxin accumulates in the periplasm ofBordetella pertussisprior to secretion, and we examined its fate following treatment with antimicrobial agents. Both antibiotics that inhibit protein synthesis (erythromycin and chloramphenicol), transcription (rifampin), or cell wall biosynthesis (cefoperazone and piperacillin) and magnesium sulfate (which inhibits transcription of pertussis toxin, but not bacterial growth) did not prevent release of preformed toxin. In contrast, agents that affect bacterial membranes, such as polymyxin B, lidocaine, procaine, and ethanol, inhibited release of preformed pertussis toxin. These results suggest new protein synthesis is not required for pertussis toxin secretion, but a functional membrane complex is required.