Exploiting advances in imaging technology to study biofilms by applying multiphoton laser scanning microscopy as an imaging and manipulation tool.
Exploiting advances in imaging technology to study biofilms by applying multiphoton laser scanning microscopy as an imaging and manipulation tool.
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通过应用多光子激光扫描显微镜作为成像和操作工具，利用成像技术的进步来研究生物膜。
DOI：
10.1111/j.1365-2818.2009.03190.x
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发表时间：
2009
影响因子：
2
通讯作者：
Lakins MA
中科院分区：
文献类型：
--
作者：
Lakins MA
生物膜是自然生态系统的重要组成部分，但在医疗保健和工业环境中可能是有害的。为了提高我们对抗生物膜的能力，我们需要了解促进其形成和分散的过程。一种已被证明是无价的方法是在生物膜生长时对其进行成像。在这里，我们描述的工具和协议，可视化生物膜与多光子激光扫描显微镜，比较这与单光子激光扫描共聚焦显微镜和突出最佳的工作程序。此外，我们描述了如何与多光子激光扫描显微镜的激光可以用来操纵生物膜，特别是实现局部漂白，杀死或消融内的生物膜生物量。这些应用为研究生物膜形成、再生和扩散的动力学开辟了新的途径。
Biofilms are an important element of the natural ecosystems but can be detrimental in health care and industrial settings. To improve our ability to combat biofilms, we need to understand the processes that facilitate their formation and dispersal. One approach that has proven to be invaluable is to image biofilms as they grow. Here we describe tools and protocols to visualize biofilms with multiphoton laser scanning microscopy, compare this with single photon laser scanning confocal microscopy and highlight best working procedures. Furthermore, we describe how with multiphoton laser scanning microscopy the laser can be used to manipulate the biofilm, specifically to achieve localized bleaching, killing or ablation within the biofilm biomass. These applications open novel ways to study the dynamics of biofilm formation, regeneration and dispersal.