Distinct UV-B and UV-A blue light signal transduction pathways induce chalcone synthase gene expression in Arabidopsis cells
Distinct UV-B and UV-A blue light signal transduction pathways induce chalcone synthase gene expression in Arabidopsis cells
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DOI：
10.1105/tpc.8.9.1555
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发表时间：
1996-09-01
期刊：
影响因子：
11.6
通讯作者：
Jenkins, GI
中科院分区：
文献类型：
--
作者：
Christie, JM;Jenkins, GI
紫外线和蓝光控制着一系列高等植物中类黄酮生物合成基因的表达。为了研究UV-B和UV-A/蓝光诱导查尔酮合酶（CHS）基因表达的信号转导过程，我们在光混合营养的拟南芥细胞悬浮培养中研究了哺乳动物系统中已知信号组分的特异性激动剂和抑制剂的作用。CHS表达在细胞培养物中由这些波长特异性诱导，其方式类似于成熟拟南芥叶组织中的方式。UV-B和UV-A/蓝色光转导过程都涉及钙，尽管细胞溶质钙的升高本身不足以刺激CHS表达。UV-A/蓝光诱导CHS表达似乎不涉及钙调蛋白，而UV-B的反应，这种差异表明，信号转导途径是，至少在部分，不同的。我们提供的证据表明，这两种途径涉及可逆的蛋白质磷酸化，并需要蛋白质合成。因此，UV-B和UV-A/蓝光信号传导途径不同于其他物种中调节CHS表达的光敏色素信号传导途径。
UV and blue light control the expression of flavonoid biosynthesis genes in a range of higher plants. To investigate the signal transduction processes involved in the induction of chalcone synthase (CHS) gene expression by UV-B and UV-A/blue light, we examined the effects of specific agonists and inhibitors of known signaling components in mammalian systems in a photomixotrophic Arabidopsis cell suspension culture. CHS expression is induced specifically by these wavelengths in the cell culture, in a manner similar to that in mature Arabidopsis leaf tissue. Both the UV-B and UV-A/blue phototransduction processes involve calcium, although the elevation of cytosolic calcium is insufficient on its own to stimulate CHS expression. The UV-A/blue light induction of CHS expression does not appear to involve calmodulin, whereas the UV-B response does; this difference indicates that the signal transduction pathways are, at least in part, distinct. We provide evidence that both pathways involve reversible protein phosphorylation and require protein synthesis. The UV-B and UV-A/blue light signaling pathways are therefore different from the phytochrome signal transduction pathway regulating CHS expression in other species.