Comparative toxic effects of butylparaben sodium, sodium diacetate and potassium sorbate to Dunaliella tertiolecta and HL7702 cells.
Comparative toxic effects of butylparaben sodium, sodium diacetate and potassium sorbate to Dunaliella tertiolecta and HL7702 cells.
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DOI：
10.1039/c7fo01102d
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发表时间：
2017-12
期刊：
影响因子：
6.1
通讯作者：
Hao-Hong Chen;Xi-lin Xu;Yu Shang;Jian-Guo Jiang
中科院分区：
文献类型：
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作者：
Hao-Hong Chen;Xi-lin Xu;Yu Shang;Jian-Guo Jiang
对羟基苯甲酸丁酯钠（BP）、双乙酸钠（SDA）和山梨酸钾（PS）是安全且国际公认的防腐剂。本研究的目的是进一步评估其毒性使用微藻细胞，并与它们的哺乳动物细胞毒性进行比较。以单细胞杜氏藻（Dunaliella tertiolecta）为实验材料，研究了BP、SDA和PS的毒性。结果表明，三种防腐剂对D.藻类的叶绿素和类胡萝卜素含量、活藻细胞以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性大幅下降。SDA和PS对正常人HL 7702肝细胞的作用较小，而BP对人乳腺癌MCF-10A细胞的作用与之相似。HL 7702的有效浓度（EC 50）值为215.97 mg L-1。BP、SDA和PS对D.在微碱性条件下，SDA具有中等毒性，PS具有高毒性。在微碱性条件下，藻细胞的敏感性高于HL 7702细胞，在酸性条件下更敏感。D. tertiolecta可用作毒性测试的预筛选。
Butylparaben sodium (BP), sodium diacetate (SDA) and potassium sorbate (PS) are safe and internationally recognized preservatives. The aim of this study is to further evaluate their toxicities using microalgae cells, and a comparison is made with their mammalian cell cytotoxicities. Unicellular Dunaliella tertiolecta, was employed to test the possible toxicities of BP, SDA and PS. The results show that the three preservatives have a negative effect on D. tertiolecta, as manifested by a strong decrease in chlorophyll and carotenoid content, viable algal cells, and superoxide dismutase (SOD) and catalase (CAT) activities. SDA and PS had small effects on the normal hepatocytes HL7702, but similar to that for MCF-10A cells, BP is toxic. The effective concentration (EC50) value for HL7702 is 215.97 mg L-1. It is concluded that BP, SDA and PS have low toxicities to D. tertiolecta under slightly alkaline conditions, while under acidic conditions, SDA has moderate toxicity and PS has high toxicity. The sensitivity of algal cells is higher than that of HL7702 cells under slightly alkaline conditions, and is even more sensitive under acidic conditions. D. tertiolecta can be used as a pre-screen for toxicity testing.