Modified expression of vitamin D receptor and CYP27B1 in denervation-induced muscle atrophy
Modified expression of vitamin D receptor and CYP27B1 in denervation-induced muscle atrophy
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DOI：
10.1016/j.bbrc.2020.05.205
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发表时间：
2020-08-27
影响因子：
3.1
通讯作者：
Fujita, Satoshi
中科院分区：
文献类型：
--
作者：
Mori, Risako;Yokokawa, Takumi;Fujita, Satoshi
维生素D途径与骨骼肌的质量和功能有关。一些研究已经证明了维生素D受体（VDR）和CYP 27 B1在骨骼肌中的作用，表明这些蛋白质可能调节骨骼肌及其功能。然而，目前尚不清楚VDR和CYP 27 B1的表达是否在骨骼肌萎缩中被改变。我们研究了去神经诱导的肌肉萎缩是否与小鼠骨骼肌中VDR和CYP 27 B1表达的改变有关。在小鼠经历去神经手术后3天和7天，从C57 BL/6 J小鼠切除骨骼肌。失神经支配导致肌肉萎缩，并增强腓肠肌和比目鱼肌中MuRF 1和Atrogin-1的表达。去神经腓肠肌VDR蛋白表达增加;相反，去神经腓肠肌和比目鱼肌CYP 27 B1蛋白表达减少。这些结果表明，去神经诱导的肌肉萎缩与小鼠骨骼肌中维生素D相关蛋白表达的变化有关。(C)2020爱思唯尔公司All rights reserved.
The vitamin D pathway is related to the mass and function of skeletal muscles. Several studies have demonstrated the role of vitamin D receptor (VDR) and CYP27B1 in skeletal muscles, suggesting that these proteins may regulate skeletal muscles and their function. However, it remains unclear whether the expression of VDR and CYP27B1 is modified in skeletal muscle atrophy. We investigated whether denervation-induced muscle atrophy is associated with altered expression of VDR and CYP27B1 in murine skeletal muscles. Skeletal muscles were excised from C57BL/6J mice, 3 and 7 days after the mice underwent denervation surgery. Denervation induced muscle atrophy and enhanced the expression of MuRF1 and Atrogin-1 in the gastrocnemius and soleus. The protein expression of VDR was increased in the denervated gastrocnemius; in contrast, denervation decreased the protein expression of CYP27B1 in the gastrocnemius and soleus. These results suggest that denervation-induced muscle atrophy is associated with changes in the expression of vitamin D-related proteins in murine skeletal muscles. (C) 2020 Elsevier Inc. All rights reserved.