Memory and naive B-cell subsets in patients with multiple sclerosis
Memory and naive B-cell subsets in patients with multiple sclerosis
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DOI：
10.1016/j.neulet.2009.08.010
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发表时间：
2009-10-16
影响因子：
2.5
通讯作者：
Sasaki, Hidenao
中科院分区：
文献类型：
--
作者：
Niino, Masaaki;Hirotani, Makoto;Sasaki, Hidenao
记忆和幼稚B细胞被认为在免疫调节中发挥不同的作用。然而，记忆和幼稚B细胞亚群在多发性硬化症（MS）中的作用尚未阐明。在这项研究中，我们检查了MS患者和健康受试者之间的记忆和幼稚B细胞亚群是否存在差异，以及干扰素β（IFN β）-1b可以影响MS患者的这些亚群。我们还研究了MS复发和缓解期的这些亚群。受试者包括31例缓解期的复发-缓解型MS患者，其中15例用IFN β-1b治疗，16例未治疗，22例健康对照。对于16名未经治疗的患者中的11名，也在复发阶段获得血液样品。流式细胞术检测记忆和幼稚B细胞中CD 5、CD 80、CD 86、CCR 5、CXCR 3、CD 11 a和CD 49 d的表达。未治疗患者的幼稚B细胞亚群中CD 86（+）细胞和CCR 5（+）细胞的百分比显著高于对照受试者或IFN β治疗患者。在MS患者中，缓解期幼稚B细胞亚群中的CD 86（+）细胞和CCR 5（+）细胞的百分比以及记忆B细胞亚群中的CD 5（+）细胞的百分比显著高于复发期。这些结果表明，记忆和幼稚B细胞亚群，尤其是CD 86（+）幼稚B细胞、CCR 5（+）幼稚B细胞和CD 5（+）记忆B细胞，可能有助于研究MS的发病机制和治疗。（C）2009 Elsevier爱尔兰Ltd.保留所有权利。
Memory and naive B cells are considered to play distinct roles in immune regulation. However, the roles of memory and naive B-cell subsets in multiple sclerosis (MS) have not yet been elucidated. In this study, we examined whether memory and naive B-cell subsets differ between patients with MS and healthy subjects and whether interferon beta (IFN beta)-1b can affect these subsets in patients with MS. We also studied these subsets in relapsing and remitting stages of MS. Subjects included 31 patients with relapsing-remitting MS in the remitting stage, of which 15 were treated with IFN beta-1b and 16 were not treated, and 22 healthy control subjects. For 11 of the 16 untreated patients, blood samples were also obtained in the relapsing stage. Expression of CD5, CD80, CD86, CCR5, CXCR3, CD11a, and CD49d in memory and naive B cells in blood samples was examined by flow cytometry. The percentages of CD86(+) cells and CCR5(+) cells in the naive B-cell subset were significantly higher in untreated patients than in control subjects or IFN beta-treated patients. In patients with MS, the percentages of CD86(+) cells and CCR5(+) cells in the naive B-cell subset and the percentage of CD5(+) cells in the memory B-cell subset were significantly greater in the remitting stage than in the relapsing stage. These results indicate that memory and naive B-cell subsets, especially CD86(+) naive B cells, CCR5(+) naive B cells, and CD5(+) memory B cells, might be useful in the study of the pathogenesis of and therapy for MS. (C) 2009 Elsevier Ireland Ltd. All rights reserved.