Prevention of the induction of allospecific cytotoxic T lymphocyte and delayed-type hypersensitivity responses by ultraviolet irradiation of corneal allografts.
Prevention of the induction of allospecific cytotoxic T lymphocyte and delayed-type hypersensitivity responses by ultraviolet irradiation of corneal allografts.
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预防同种异体角膜移植物紫外线照射诱导同种异体特异性细胞毒性 T 淋巴细胞和迟发型超敏反应。
DOI：
10.1097/00007890-199008000-00023
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发表时间：
1990
期刊：
影响因子：
6.2
通讯作者：
Ross,JR
中科院分区：
文献类型：
--
作者：
Niederkorn,JY;Callanan,D;Ross,JR
在小鼠角膜移植模型上观察了紫外线照射（UVR）对角膜移植免疫原性的影响。异于宿主在整个MHC和多个次要H位点的角膜同种异体移植物进行了200 mJ/cm 3的UVB照射前立即异位移植。细胞毒性T淋巴细胞和迟发型超敏反应的分析表明，UVR处理的角膜移植不能诱导C57 BL/6受体的CTL或DTH反应。UVB治疗消除了含有常驻或浸润供体特异性朗格汉斯细胞的高免疫原性角膜移植物的免疫原性。连续移植实验表明，UVB处理的移植物呈现出的主机无反应，随后免疫与高免疫原性角膜利姆布斯移植物，含有密集浓度的Ia+朗格汉斯细胞的供体来源。结果表明，紫外线处理不仅降低了角膜移植物的免疫原性，但也可能使其耐受原性。
The effect of ultraviolet radiation (UVR) on the immunogenicity of corneal allografts was examined in a mouae model. Corneal allografts differing from the host at the entire MHC and multiple minor H loci were subjected to 200 mJ/cm 3 of UVB irradiation immediately prior to heterotopic transplantation. Analysis of cytotoxic T lymphocyte and delayed-type hypersensitivity responses revealed that UVR treated corneal grafts failed to induce either CTL or DTH responses in C57BL/6 recipients. UVB treatment abolished the immunogenicity of highly immunogenic corneal grafts containing either resident or infiltrating donor-specific Langerhans cells. Sequential grafting experiments demonstrated that UVB-treated grafts rendered the hosts anergic to subsequent immunization with highly immunogenic corneal limbus grafts that contained dense concentrations of Ia+ Langerhans cells of donor origin. The results indicate that UV treatment not only reduces the immunogenicity of the corneal allograft but may also render it tolerogenic.