Comparative study on protective effect of hydrogen rich saline and adipose-derived stem cells on hepatic ischemia-reperfusion and hepatectomy injury in swine
Comparative study on protective effect of hydrogen rich saline and adipose-derived stem cells on hepatic ischemia-reperfusion and hepatectomy injury in swine
复制标题
富氢盐水与脂肪干细胞对猪肝脏缺血再灌注及肝切除损伤保护作用的比较研究
DOI：
10.1016/j.biopha.2019.109453
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发表时间：
2019
影响因子：
7.5
通讯作者：
Wang Hongbin
中科院分区：
文献类型：
--
作者：
Zhang Qianzhen;Piao Chenxi;Xu Jiayuan;Jiao Zhihui;Ge Yansong;Liu Xiaoning;Ma Yajun;Wang Hongbin
目的比较和评价肝实质注射ADSCs和门静脉注射HRS对腹腔镜下肝缺血再灌注合并肝切除损伤小型猪的保护作用。分别于再灌注前10 、术后1 d、2 d、3 d经门静脉注射HRS。肝切除后向肝实质内注射ADSCs。分别于术前、术后1 d、3 d和7 d采集血清和肝组织标本。结果与IR组相比，ADSCs组和HRS组均能促进肝功能恢复，减轻氧化应激，减轻炎症反应，促进肝再生。术后3 d，ADSCs组ALT、TBIL显著降低，1 d，AST显著降低，1 d，ADSCs组超氧化物歧化酶、谷胱甘肽过氧化物酶活性显著高于HRS组，而丙二醛水平显著低于ADSCs组，术后1 d，ADSCs组IL-1β水平显著低于HRS组。结论HRS和ADSCs对小型猪HIRI和肝切除术后的肝损伤有明显的保护作用。肝实质注射ADSCs比门静脉注射HRS更有利于肝功能的恢复。
AimTo compare and evaluate the hepatoprotective effect of liver parenchyma injection of ADSCs and portal vein injection of HRS in laparoscopic hepatic ischemia reperfusion combined with hepatectomy injury in miniature pigs.MethodsEighteen miniature pigs were randomly assigned to IRI group, HRS group and ADSCs group. HRS was injected through the portal vein 10 min before reperfusion, 1 d, 2 d, and 3 d after surgery. ADSCs were injected into liver parenchyma after hepatectomy. The serum and liver tissue samples were collected at different time points (preoperative, and postoperative at 1 d, 3 d and 7 d).ResultsCompared with the IRI group, both ADSCs and HRS groups can promote liver function recovery, reduce oxidative stress, reduce inflammation, and promote liver regeneration. Compared with HRS, ALT and TBIL in ADSCs group were significantly decreased at 3 d, and AST was significantly reduced at 1 d. The activities of SOD and GSH-Px in ADSCs group were significantly higher than that in HRS group, but the MDA level in HRS group was markedly lower than that in ADSCs group at 1 d. IL-1β was significantly lower in the ADSCs group than in the HRS group at 1 day after operation. The expressions of HGF and PCNA were significantly higher than that in the HRS group at 3 day after surgery.ConclusionOur study has demonstrated that HRS and ADSCs have significant hepatoprotective effects in miniature pigs after HIRI and hepatectomy injury. However, liver parenchyma injection of ADSCs is more beneficial to the recovery of liver function than portal vein injection of HRS.