Steady-state kinetics of serum bile acids in healthy human subjects: single and dual isotope techniques using stable isotopes and mass spectrometry.
Steady-state kinetics of serum bile acids in healthy human subjects: single and dual isotope techniques using stable isotopes and mass spectrometry.
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发表时间：
1987-03
影响因子：
6.5
通讯作者：
G. Everson
中科院分区：
文献类型：
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作者：
G. Everson
建立了用稳定同位素和毛细管气-液质谱(GLC-MS)测定健康受试者血清中鹅去氧胆酸(CDCA)和胆酸(CA)的完全稳态动力学和脱氧胆酸(DCA)池大小的技术。血清胆汁酸的纯化采用C18色谱柱、酸解、酶解、甲基化、C8色谱柱和TMS-乙醚衍生化的方法。5名健康受试者分别给予[24-13C]CDCA和[24-13C]CA各50 mg，测定血清和胆汁的血药浓度。在每个病例中，来自血清(S)的测量结果与来自胆汁(B)的测量结果相同(CDCA(S vs.B)：分数周转率(FTR)(d-1)0.17+/-0.03vs.0.18+/-0.04；池(G)0.64+/-0.1vs.0.68+/-0.14；合成(gd-1)0.12+/-0.03vs.0.1+/-0.03；CA(S对B)：FTR(d-1)0.28+/-0.05对0.29+/-0.07，池(G)0.84+/-0.29对0.82+/-0.29，合成(g d-1)0.24+/-0.10对0.25+/-0.12)。此外，还建立了用[11，12-2H]CDCA、[24-13C]CDCA和单个血清样品测定CDCA稳态动力学的双同位素技术。10例受试者血清和胆汁测得的FTR、CDCA池和CDCA合成量相似。最后，建立了一种用脱氧胆酸(DCA)的370个离子与CDCA的370个离子的比值来估计血清中DCA库的方法。综上所述，这些数据表明，CDCA和CA的稳态动力学和DCA池的大小可以从健康人的血清中测量出来。
Techniques have been developed for the measurement of the complete steady-state kinetics of both chenodeoxycholic (CDCA) and cholic (CA) acid and the pool size of deoxycholic acid (DCA) from the serum of healthy subjects using stable isotopes and capillary gas-liquid chromatography-mass spectrometry (GLC-MS). Serum bile acids were purified by a method employing a C18 chromatographic cartridge, acid solvolysis, enzymic hydrolysis, methylation, a C8 chromatographic cartridge, and TMS-ether derivatization. Fifty mg each of [24-13C]CDCA and [24-13C]CA was given to five healthy subjects and kinetics were measured from serum and bile. In each case, the measurements from serum (S) equalled those from bile (B) (CDCA (S vs. B): fractional turnover rate (FTR) (d-1) 0.17 +/- 0.03 vs. 0.18 +/- 0.04; pool (g) 0.64 +/- 0.1 vs. 0.68 +/- 0.14, synthesis (g d-1) 0.12 +/- 0.03 vs. 0.1 +/- 0.03; CA (S vs. B): FTR (d-1) 0.28 +/- 0.05 vs. 0.29 +/- 0.07, pool (g) 0.84 +/- 0.29 vs. 0.82 +/- 0.29, synthesis (g d-1) 0.24 +/- 0.10 vs. 0.25 +/- 0.12). In addition, a dual isotope technique for measuring the steady-state kinetics of CDCA was developed using [11,12-2H]CDCA, [24-13C]CDCA, and a single sample of serum. In ten subjects, the FTR, pool and synthesis of CDCA measured from serum was similar to that measured from bile. Finally, a technique for estimating the deoxycholic acid (DCA) pool from serum using the ratio of the 370 ion of DCA to that of CDCA was developed. In summary, these data demonstrate that the steady-state kinetics of CDCA and CA and the pool size of DCA can be measured from the serum of healthy subjects.