Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov.
Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov.
复制标题
DOI：
10.1309/731v-vgvc-kk35-1y4j
复制
发表时间：
2003-08
影响因子：
3.5
通讯作者：
Xiang Y. Han;A. S. Pham;J. Tarrand;K. Rolston;L. Helsel;P. Levett
中科院分区：
文献类型：
--
作者：
Xiang Y. Han;A. S. Pham;J. Tarrand;K. Rolston;L. Helsel;P. Levett
我们使用了多相的方法（16 S核糖体RNA基因的测序分析和表型分析），以表征36株玫瑰单胞菌属物种从血液中分离。以菌株MDA 5176（M.D.安德森癌症中心），被鉴定为玫瑰单胞菌gilardii。其中1株为玫瑰单胞菌基因种4。以菌株MDA 5527为代表的22株菌株与吉氏红单胞菌和其他玫瑰单胞菌属的菌株在基因型和表型上均存在显著差异，代表一个新的玫瑰单胞菌属;粘膜玫瑰单胞菌新种（Roseomonasmucosissp nov）被认为是其突出的粘液状、几乎流鼻涕的菌落。以菌株MDA 5605为代表的8株菌与吉氏玫瑰单胞菌差异较小，菌落呈明显的粉红色至红色，建议将其命名为吉氏玫瑰单胞菌玫瑰亚种新亚种。对于亚种分化，R gilardii被建议为R gilardii subsp gilardii subsp nov。这些玫瑰单胞菌属的物种，这可能有助于这些生物体的常规鉴定建立独特的生化反应模式。所有36株和2株美国典型培养物保藏中心菌株对阿米卡星和环丙沙星敏感，而对头孢吡肟和头孢他啶耐药。他们也经常对亚胺培南和替卡西林-克拉维酸敏感，但对头孢曲松、甲氧苄啶-磺胺甲恶唑和氨苄西林的敏感性要低得多。粘膜R菌株最耐药，而R gilardii subsp gilardii菌株最敏感。
We used a polyphasic approach (sequencing analysis of the 16S ribosomal RNA gene and phenotypic analyses) to characterize 36 strains of Roseomonas species isolated from blood. Five strains, represented by strain MDA5176 (M.D. Anderson Cancer Center), were identified as Roseomonas gilardii. One strain belonged to Roseomonas genomospecies 4. The 22 strains represented by strain MDA5527 showed significant differences genotypically and phenotypically with R gilardii and other Roseomonas species and represented a new Roseomonas species; Roseomonas mucosa sp nov was proposed to denote its prominent mucoid, almost runny colonies. Eight strains, represented by strain MDA5605, had minor differences with R gilardii and displayed obvious pink to red colonies; Roseomonas gilardii subsp rosea subsp nov was proposed. For subspecies differentiation, R gilardii was proposed to be R gilardii subsp gilardii subsp nov. Unique patterns of biochemical reactions were established for these Roseomonas species, which may assist routine identification of these organisms. All 36 strains and 2 American Type Culture Collection strains were susceptible to amikacin and ciprofloxacin but resistant to cefepime and ceftazidime. They also were frequently susceptible to imipenem and ticarcillin-clavulanate but far less susceptible to ceftriaxone, trimethoprim-sulfamethoxazole, and ampicillin. R mucosa strains were most resistant, whereas R gilardii subsp gilardii strains were most susceptible.