Adenosine stimulates phosphate and glucose transport in opossum kidney epithelial cells.
Adenosine stimulates phosphate and glucose transport in opossum kidney epithelial cells.
复制标题
腺苷刺激负鼠肾上皮细胞中的磷酸盐和葡萄糖转运。
DOI：
10.1152/ajprenal.1991.260.6.f921
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Scheinman,SJ
中科院分区：
文献类型：
--
作者：
Coulson,R;Johnson,RA;Olsson,RA;Cooper,DR;Scheinman,SJ
我们研究了腺苷对培养负鼠肾（OK）细胞中钠偶联磷酸盐和葡萄糖运输的影响，负鼠肾是一种类似近端小管的连续细胞系。腺苷类似物R-(-)- n6 -苯异丙腺苷（PIA）和2',5'-二脱氧腺苷（DDA）分别作为腺苷A1受体和P位点选择性激动剂。在PIA（0.1, 1微米）处理的细胞中，钠依赖性磷酸盐摄取活性（Na-Pi同向性）比基础和甲状旁腺激素（PTH）抑制水平增加了约25%，但在DDA（100微米）处理的细胞中没有。腺苷（PIA）也刺激了约40%的钠偶联3- o -甲基葡萄糖运输。经PIA和PTH处理的细胞内腺苷3′，5′-环单磷酸腺苷（cAMP）含量与Na-Pi同调活性呈负相关。然而，Na-Pi交感神经活性的变化与细胞内cAMP的变化并不一致。蛋白激酶C在1 μ m PIA作用于OK细胞15 s后被激活。用3微米的星孢素预孵育细胞可减弱1微米的PIA对磷酸盐摄取的影响。这些数据表明，Na-Pi和na -葡萄糖同调活性是由腺苷作用于一个受体偶联到一个以上的细胞内信号而刺激的。很可能蛋白激酶A和C都参与了腺苷的这些作用。
We have examined the effects of adenosine on sodium-coupled phosphate and glucose transport in cultured opossum kidney (OK) cells, a continuous cell line that resembles proximal tubule. Adenosine analogues R-(-)-N6-phenylisopropyladenosine (PIA) and 2',5'-dideoxy-adenosine (DDA) were employed as adenosine A1 receptor and P site-selective agonists, respectively. Sodium-dependent phosphate uptake activity (Na-Pi symport) increased by approximately 25% above both basal and parathyroid hormone (PTH)-inhibited levels in cells treated with PIA (0.1, 1 microM) but not in cells treated with DDA (100 microM). Adenosine (PIA) also stimulated sodium-coupled 3-O-methylglucose transport by approximately 40%. Intracellular adenosine 3',5'-cyclic monophosphate (cAMP) content was inversely related to Na-Pi symport activity in cells treated with PIA and PTH. However, changes in Na-Pi symport activity did not consistently relate to changes in intracellular cAMP. Protein kinase C was activated 15 s after treatment of OK cells with 1 microM PIA. Preincubation of cells with 3 microM staurosporine attenuated the effect of 1 microM PIA on phosphate uptake. These data suggest that Na-Pi and Na-glucose symport activities are stimulated by adenosine acting at a receptor coupled to more than one intracellular signal. It is likely that both protein kinases A and C are involved in these actions of adenosine.