Detecting Reactive Oxygen Species Generation and Stem Cell Proliferation in the Drosophila Intestine
Detecting Reactive Oxygen Species Generation and Stem Cell Proliferation in the Drosophila Intestine
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DOI：
10.1007/978-1-4939-3603-8_10
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Jones, Rheinallt M.
中科院分区：
文献类型：
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作者：
Luo, Liping;Reedy, April R.;Jones, Rheinallt M.
果蝇和哺乳动物之间肠道干细胞隐窝动态的保守性使得遗传易驯化的果蝇模型可用于分析与微生物群相关的肠道发育、稳态和更新。无脊椎动物果蝇模型由于其解剖和遗传简单且寿命短，因此有利于遗传学研究。因此，开发了大量突变和转基因果蝇等实验资源。我们开发了技术来产生无菌果蝇，将它们与候选细菌单结合，并评估肠道组织内随后的生理反应，包括活性氧的产生和细胞增殖。
The conservation of intestinal stem cell crypt dynamics between Drosophila melanogaster and mammals allows for the genetically tractable fly model to be used for analyses of intestinal development, homeostasis, and renewal in relation to microbiota. The invertebrate fly model is advantageous for genetic research due to its anatomical and genetic simplicity and short lifespan. Accordingly, experimental resources such as large numbers of mutant and genetically modified flies have been developed. We have developed techniques to generate germ-free Drosophila, monoassociate them with candidate bacteria, and assess ensuing physiological responses within the gut tissue that include the generation of reactive oxygen species and cell proliferation.