Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer.
Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer.
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DOI：
10.1063/1.4878696
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发表时间：
2014-05
期刊：
影响因子：
3.2
通讯作者：
I. Katis;J. Holloway;J. Madsen;S. Faust;S. Garbis;Peter J. Smith;D. Voegeli;D. Bader;R. Eason;C. Sones
中科院分区：
文献类型：
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作者：
I. Katis;J. Holloway;J. Madsen;S. Faust;S. Garbis;Peter J. Smith;D. Voegeli;D. Bader;R. Eason;C. Sones
我们报告了抗体从液体供体膜到纸接收器上的激光诱导前向转移（LIFT），用于即时诊断传感器。为了使转移过程中活性生物分子的功能损失最小化，采用动态释放层来保护生物材料免于直接暴露于脉冲激光源。选择纤维素纸作为理想的接收器是因为其固有的生物相容性、液体传输特性、广泛的可用性和低成本，所有这些使其成为即时诊断传感器的有效和合适的平台。酶标记和未标记的IgG抗体都进行了LIFT打印，并通过比色酶联免疫吸附测定法确认了它们的功能。展示了打印抗体的局部化，这可以允许创建复杂的二维图案，如QR码或字母，用于最终的工作设备。最后，确定校准曲线，其将获得的颜色强度与活性抗体的浓度相关联，以能够定量评估装置性能。这项工作的动机是实施一种基于激光的程序，用于在纸上制造低成本的即时诊断设备。
We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care diagnostic sensors. To minimise the loss of functionality of the active biomolecules during transfer, a dynamic release layer was employed to shield the biomaterial from direct exposure to the pulsed laser source. Cellulose paper was chosen as the ideal receiver because of its inherent bio-compatibility, liquid transport properties, wide availability and low cost, all of which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed and their functionality was confirmed via a colorimetric enzyme-linked immunosorbent assay. Localisation of the printed antibodies was exhibited, which can allow the creation of complex 2-d patterns such as QR codes or letters for use in a final working device. Finally, a calibration curve was determined that related the intensity of the colour obtained to the concentration of active antibodies to enable quantitative assessment of the device performance. The motivation for this work was to implement a laser-based procedure for manufacturing low-cost, point-of-care diagnostic devices on paper.