Antimicrobial Resistance in Generic Escherichia coli Isolates from Wild Small Mammals Living in Swine Farm, Residential, Landfill, and Natural Environments in Southern Ontario, Canada
Antimicrobial Resistance in Generic Escherichia coli Isolates from Wild Small Mammals Living in Swine Farm, Residential, Landfill, and Natural Environments in Southern Ontario, Canada
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DOI：
10.1128/aem.01111-10
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发表时间：
2011-02-01
影响因子：
4.4
通讯作者：
Jardine, Claire
中科院分区：
文献类型：
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作者：
Allen, Samantha E.;Boerlin, Patrick;Jardine, Claire
为了评估不同类型的人类活动对野生小型哺乳动物粪便中耐药细菌发展的影响，我们比较了从生活在四种环境中的野生小型哺乳动物粪便样本中采集的大肠杆菌和沙门氏菌分离株的抗菌素耐药性和耐药基因的流行率和模式：猪场，居民区，垃圾填埋场和自然栖息地。大肠杆菌对抗菌药物耐药。大肠杆菌从所有环境中的动物分离：25/52（48%）的动物被困在猪场，6/69（9%）的动物被困在住宅区，3/20（15%）的动物被困在垃圾填埋场，1/22（5%）的动物被困在自然栖息地。被困在农场的动物携带E。大肠杆菌分离物对四环素、氨苄青霉素、磺胺异恶唑和链霉素的耐药性高于居民区捕获的动物。sul 2、aadA和泰特（A）基因在E.从农场捕获的动物中分离出的大肠杆菌比从住宅区捕获的动物中分离出的大肠杆菌要多。三个S从4/302（1%）野生小型哺乳动物的粪便中回收肠道菌血清型（Give、Typhimurium和纽波特）。所有沙门氏菌分离株均为泛易感。我们的研究结果表明，猪场的起源是显着相关的存在耐药细菌和耐药基因在野生小哺乳动物在加拿大南部安大略。然而，在所有研究环境中生活的小型哺乳动物中发现了耐药粪便细菌，表明环境暴露于抗菌剂，抗菌剂残留物，耐药细菌或耐药基因是普遍存在的。
To assess the impacts of different types of human activity on the development of resistant bacteria in the feces of wild small mammals, we compared the prevalences and patterns of antimicrobial resistance and resistance genes in generic Escherichia coli and Salmonella enterica isolates from fecal samples collected from wild small mammals living in four environments: swine farms, residential areas, landfills, and natural habitats. Resistance to antimicrobials was observed in E. coli isolates from animals in all environments: 25/52 (48%) animals trapped at swine farms, 6/69 (9%) animals trapped in residential areas, 3/20 (15%) animals trapped at landfills, and 1/22 (5%) animals trapped in natural habitats. Animals trapped on farms were significantly more likely to carry E. coli isolates with resistance to tetracycline, ampicillin, sulfisoxazole, and streptomycin than animals trapped in residential areas. The resistance genes sul2, aadA, and tet(A) were significantly more likely to be detected in E. coli isolates from animals trapped on farms than from those trapped in residential areas. Three S. enterica serotypes (Give, Typhimurium, and Newport) were recovered from the feces of 4/302 (1%) wild small mammals. All Salmonella isolates were pansusceptible. Our results show that swine farm origin is significantly associated with the presence of resistant bacteria and resistance genes in wild small mammals in southern Ontario, Canada. However, resistant fecal bacteria were found in small mammals living in all environments studied, indicating that environmental exposure to antimicrobials, antimicrobial residues, resistant bacteria, or resistance genes is widespread.