Application of Plant Extracts for the Prevention of Dental Erosion: An in situ/in vitro Study
Application of Plant Extracts for the Prevention of Dental Erosion: An in situ/in vitro Study
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DOI：
10.1159/000431294
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发表时间：
2015-01-01
期刊：
影响因子：
4.2
通讯作者：
Hannig, Christian
中科院分区：
文献类型：
--
作者：
Weber, Marie-Theres;Hannig, Matthias;Hannig, Christian
目的：某些植物提取物的抗粘附和抗菌作用已被证明对预防性牙科有益。在目前的原位/体外交叉研究中，评估了富含多酚的植物提取物对原位表膜的侵蚀防护特性的影响。方法：为 12 名受试者准备单独的夹板，用于口腔内暴露牛牙釉质标本。形成 1 分钟的薄膜后，将水性植物提取物（野生黑醋栗的叶子、野生牛至以及两者的组合）原位施用 10 分钟。或者，用氟化物 (Elmex Kariesschutz) 漱口 1 分钟。分别进一步口服暴露 19/28 分钟后，取出平板并在体外与 HCl 一起孵育 120 秒以上（pH 2、2.3、3）。所产生的钙和磷酸盐的释放通过光度测定进行定量。有和没有 30 分钟原位薄膜的板作为对照。通过透射电子显微镜（TEM）评估薄膜超微结构的改变。结果：植物提取物以 pH 依赖性方式调节所有测试组中天然原位表膜的侵蚀防护特性。 R. nigrum 叶和牛至的组合增强了在所有 pH 值下薄膜的保护性能；该制剂的使用效果与氟化漱口水的效果相当，但更胜一筹。 TEM 图像表明，用 R. nigrum 叶/牛至树冲洗产生了明显更厚且电子密度更高的薄膜。结论：某些植物提取物的组合提供了一种补充预防牙齿侵蚀的新方法。 (C) 2015 S. Karger AG，巴塞尔
Objectives: Antiadherent and antibacterial effects of certain plant extracts have been proven to be beneficial in preventive dentistry. In the present in situ/in vitro crossover study, the impact of plant extracts rich in polyphenols on the erosion-protective properties of the in situ pellicle was evaluated. Methods: Individual splints were prepared for 12 subjects for intraoral exposure of bovine enamel specimens. Following formation of a 1-min pellicle, watery plant extracts (leaves of the wild form of Ribes nigrum, the wild form of Origanum as well as a combination of both) were administered for 10 min in situ. Alternatively, a mouth rinse with fluorides (Elmex Kariesschutz) was performed for 1 min. After further oral exposure for 19/28 min, respectively, slabs were removed and incubated with HCl in vitro over 120 s (pH 2, 2.3, 3). The resulting calcium and phosphate release was quantified photometrically. Slabs with and without a 30-min in situ pellicle served as controls. The modification of pellicle ultrastructure was evaluated by transmission electron microscopy (TEM). Results: Plant extracts modulated the erosion-protective properties of the native in situ pellicle in all test groups in a pH-dependent manner. The combination of R. nigrum leaves and Origanum enhanced the protective properties of the pellicle at all pH values; the administration of this preparation was comparable, yet superior, to the effect of the fluoridated mouth rinse. TEM images indicated that rinsing with R. nigrum leaves/Origanum yielded a distinctly thicker and more electron-dense pellicle. Conclusion: The combination of certain plant extracts offers a novel approach to the complementary prevention of dental erosion. (C) 2015 S. Karger AG, Basel